Membrane insertion of α-xenorhabdolysin in near-atomic detail
- Max Planck Institute of Molecular Physiology, Germany
- The University of Texas, United States
- Cited 5
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Abstract
α-Xenorhabdolysins (Xax) are α-pore-forming toxins (α-PFT) that form 1-1.3 MDa large pore complexes to perforate the host cell membrane. PFTs are used by a variety of bacterial pathogens to attack host cells. Due to the lack of structural information, the molecular mechanism of action of Xax toxins is poorly understood. Here, we report the cryo-EM structure of the XaxAB pore complex from Xenorhabdus nematophila and the crystal structures of the soluble monomers of XaxA and XaxB. The structures reveal that XaxA and XaxB are built similarly and appear as heterodimers in the 12-15 subunits containing pore, classifying XaxAB as bi-component α-PFT. Major conformational changes in XaxB, including the swinging out of an amphipathic helix are responsible for membrane insertion. XaxA acts as an activator and stabilizer for XaxB that forms the actual transmembrane pore. Based on our results, we propose a novel structural model for the mechanism of Xax intoxication.
Article and author information
Author details
Funding
Max-Planck-Gesellschaft (Open-access funding)
- Stefan Raunser
European Commission
- Stefan Raunser
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Olga Boudker, Weill Cornell Medicine, United States
Publication history
- Received: May 2, 2018
- Accepted: July 15, 2018
- Accepted Manuscript published: July 16, 2018 (version 1)
- Version of Record published: August 10, 2018 (version 2)
Copyright
© 2018, Schubert et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Further reading
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- Microbiology and Infectious Disease
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- Immunology and Inflammation
- Microbiology and Infectious Disease
