(A) Dorsal view of P21 posterior brains, scale bar 2 mm. (B) Quantification of the cerebellar anterior-posterior and medio-lateral axes (n = 4 for each genotype), **p-value=0.0018. (C) Representative images of hematoxylin-eosin (HE) stained P21 sagittal slices of control and mutant cerebella, scale bar 1 mm. Lobule numbers are indicated. (D) Quantification of the midline sagittal cerebellar area (Control, n = 5; En1-Cre; Srd5a3fl/-, n = 4); *p-value=0.0245. (E) Magnification of HE-stained cerebellar cortex, scale bar 200 μm. All examined mutants show ectopic cell clusters (arrow-head) in the outer part of the molecular layer (ML). (F) DAPI (blue) and NeuN (red) staining of P21 En1-Cre; Srd5a3fl/- cerebellum. Ectopic cells (arrow-head) are positive for the post-mitotic GCs marker NeuN, scale bar 50 μm. (G) Representative image of an HE-stained sagittal slice of Atoh1-Cre; Srd5a3fl/- cerebellum showing similar ectopic cells in the outer ML under GC-specific Srd5a3 deletion, scale bar 1 mm and 100 μm, respectively (n = 4). Two-tailed Student t-test was used for statistics. *p<0.05; **p<0.01. Results are presented as mean ± s.d.