(A) Intersectional strategy to label only those neurons with a history of Atoh1 (FlpO) and En1 (Cre) expression. Representative whole-brain image of P21 Atoh1FlpO;En1Cre;Ai65/+ mouse on the right. (B) Cell bodies in the PBC are tdTomato+. (C) tdTomato+ puncta overlap with the synapse-marker synapsin. (D) Schematic of the respiratory circuitry. Solid red PBC is the only respiratory nucleus expressing tdTomato. Dotted nuclei are the nuclei in which tdTomato+ projections were observed. TdTomato+ puncta near cell bodies were observed in the (E) ITR, (F) RTN, and (G) preBötC. No tdTomato+puncta were observed in the (H) NTS, or in respiratory motor nuclei (I) mV, (J) mVII, (K), NA, (L), XII. Abbreviations: PBC, parabrachial complex; ITR, intertrigeminal region; RTN, retrotrapezoid nucleus; preBötC, preBötzinger Complex; NTS, nucleus tractus solitarius; mV, trigeminal motor nucleus; mVII, facial motor nucleus; NA, nucleus ambiguus; XII, hypoglossal motor nucleus.