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Thalamic reticular control of local sleep in mouse sensory cortex

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Cite this article as: eLife 2018;7:e39111 doi: 10.7554/eLife.39111

Abstract

Sleep affects brain activity globally, but many cortical sleep waves are spatially confined. Local rhythms serve cortical area-specific sleep needs and functions, however, mechanisms controlling locality are unclear. We identify the thalamic reticular nucleus (TRN) as a source for local, sensory-cortex-specific non-rapid-eye-movement sleep (NREMS) in mouse. Neurons in optogenetically identified sensory TRN sectors showed stronger repetitive burst discharge compared to non-sensory TRN cells due to higher activity of the low-threshold Ca2+ channel CaV3.3. Major NREMS rhythms in sensory but not non-sensory cortical areas were regulated in a CaV3.3-dependent manner. In particular, NREMS in somatosensory cortex was enriched in fast spindles, but switched to delta wave-dominated sleep when CaV3.3 channels were genetically eliminated or somatosensory TRN cells chemogenetically hyperpolarized. Our data indicate a previously unrecognized heterogeneity in a powerful forebrain oscillator that contributes to sensory-cortex-specific and dually regulated NREMS, enabling local sleep regulation according to use- and experience-dependence.

Article and author information

Author details

  1. Laura MJ Fernandez

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7942-3369
  2. Gil Vantomme

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-7441-0737
  3. Alejandro Osorio-Forero

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4341-4206
  4. Romain Cardis

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  5. Elidie Béard

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  6. Anita Lüthi

    Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
    For correspondence
    anita.luthi@unil.ch
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4954-4143

Funding

Swiss National Science Foundation (31003A_166318)

  • Laura MJ Fernandez
  • Gil Vantomme
  • Alejandro Osorio-Forero
  • Romain Cardis
  • Elidie Béard
  • Anita Lüthi

Etat de Vaud

  • Laura MJ Fernandez
  • Gil Vantomme
  • Alejandro Osorio-Forero
  • Romain Cardis
  • Elidie Béard
  • Anita Lüthi

FBM Poste de soutien à un congé parental

  • Laura MJ Fernandez
  • Elidie Béard

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All experimental procedures complied with the Swiss National Institutional Guidelines on Animal Experimentation (Swiss Federal Act on Animal Protection, LPA 2005) and were approved by the Swiss Cantonal Veterinary Office Committee for Animal Experimentation. All experiments were carried out in accordance with approved protocols by the Swiss Cantonal Veterinary Office Committee for in vitro experimentation on mice (reference VD2062) and for in vivo experimentation on mice (references VD2387 and VD2401).

Reviewing Editor

  1. John Huguenard, Stanford University School of Medicine, United States

Publication history

  1. Received: June 15, 2018
  2. Accepted: December 19, 2018
  3. Accepted Manuscript published: December 25, 2018 (version 1)
  4. Version of Record published: January 22, 2019 (version 2)

Copyright

© 2018, Fernandez et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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