LEAFY maintains apical stem cell activity during shoot development in the fern Ceratopteris richardii 

[Editors’ note: this article was originally rejected after discussions between the reviewers, but the authors were invited to resubmit after an appeal against the decision.]

Thank you for submitting your work entitled "LEAFY maintains apical stem cell activity during shoot development in the fern Ceratopteris richardii" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and a Senior Editor. The reviewers have opted to remain anonymous.

Our decision has been reached after consultation between the reviewers. Based on these discussions and the individual reviews below, we regret to inform you that your work will not be considered for publication in eLife in its present form.

The reviewers agreed that CrLFY2 promoter analysis and/or in situ hybridization, as well as controls for the RNAi analysis, are needed. eLife recommends a "reject" decision if it is likely that additional experiments will take more than 2 months. We therefore collectively agreed that rejection was the correct decision – however, the reviewers were all enthusiastic about the work and would strongly support re-submission to eLife if the requested experiments were included.

Reviewer #1:

This manuscript is the first to examine gene function using reverse genetics in a stably transformed fern, which is very exciting. The authors focus on LEAFY, which has been well characterized in moss and Arabidopsis. While it was previously known that the two Ceratopteris LEAFY genes are expressed in gametophytes and sporophytes, this study examines more detailed expression patterns and addresses their functions in both Ceratopteris generations by RNAi. The topic is very interesting and important in understanding fern development and plant evolution in general.

Comments/questions relating to the gene expression studies:

1) Please describe the LFY promoter more; e.g., does it include all sequences upstream of the a) transcript initiation site b) the translation start site c) second exon that would include the first intron? This may be in the details of the supplementary figures (I couldn't tell), but I think it's important to include such details in the text.

2) Any reason why LFY2 promoter-GUS expression wasn't included? The conclusion that two LFY homologs in Ceratopteris function together to control of apical cell identity would be better supported if the expression patterns of CrLFY2 were examined using the same strategy (GUS reporter).

3) I assume that a T₁ sporophyte is the product a self of a gametophyte that came from the spores of a transformed sporophyte that was selected then regenerated from bombarded callus. Is this correct?

My comments/questions relating to the RNAi lines:

1) What does phenotypic screening in the line "phenotypic screening identified 10 lines with similar developmental defects that were associated with reduced CrLFY expression" mean? My concern here is that the phenotypes described may be caused by the callus derived shoot regeneration process, which typically happens during callus regeneration in many different plant species. The authors need to carefully characterize the phenotypes of multiple individual transgenic plants together with their segregated siblings that do not contain the RNAi construct. I would also suggest performing vigorous genetic analyses to clearly define and confirm the phenotypes from multiple independent transgenic lines (i.e., backcrossing to or by wild type and examining whether the phenotype is tightly correlated to the repression of LFY genes in the F2 population). Wouldn't selection for drug resistance be useful here?

2) If some of the lines never develop gametophytes with gametangia, how were T₂ or T₃ lines generated?

My comments regarding the Discussion:

Much of this study is based on a single fern (Ceratopteris) and on one gene (LFY). For this reason, I regard the discussion of the evolutionary trajectory of LFY to be very speculative. The last sentence "if a pathway for regulating stem cell activity was co-opted from the gametophyte into the sporophyte, it was the LFY pathway" should be changed to.. if a pathway for regulating stem cell activity was co-opted from the gametophyte into the sporophyte, the pathway involving LFY is more likely than the KNOX pathway." There could be other pathways as well.

Because the Discussion focuses on evolution, it is important that this study include at least some supporting data from at least one other fern.

Reviewer #2:

Comparative analyses between bryophytes and angiosperms have been provided the information how gene function evolved to facilitate developmental innovations during land plant evolution as the authors pointed out. Ferns are one of missing link though it takes very important position during land plant evolution. Ceratopteris has been recognized as a model fern because of the early life cycle. The establishment of transformation technique in Ceratopteris became a breakthrough in this field (Muthukumar et al., 2013, Plant Physiol.; Plackett et al., 2014; Bui et al., 2015, BMC Res. Notes). LFY is one of well-studied genes among land plant lineages (e.g. Maizel et al., 2005; Sayou et al., 2014). Functional analysis of fern Ceratopteris LFY genes should be interesting topic for many readers.

The authors carefully examined the expression level of two Ceratopteris LFY genes, CrLFY1 and 2, spatial expression analysis of CrLFY1 using promoter analysis, and suppression analysis of CrLFY1 and CrLFY2 activity by RNAi. This paper will be one of model case of EvoDevo study using the fern Ceratopteris. The quality of analyses and figures is excellent, however two questions remain.

1) Why the authors did not provide the expression data by CrLFY2 promoter analysis nor in situ hybridization data of CrLFY2.

The authors indicated that CrLFY1 and CrLFY2 were differentially expressed during the Ceratopteris life cycle (subsection “CrLFY1 and CrLFY2 transcripts accumulate differentially during the Ceratopteris lifecycle”) and CrLFY1 and CrLFY2 act partially redundantly to maintain indeterminacy of the shoot apex in Ceratopteris (subsection “CrLFY1 regulates activity of the sporophyte shoot apex”). Also they discussed that the duplicate CrLFY genes therefore act at least partially redundantly during shoot development in Ceratopteris (Discussion, first paragraph). It would be very helpful to understand the difference and redundancy in the function of CrLFY1 and 2, if they have the spatial expression data of CrLFY2.

The authors described that no GUS activity was detected in unfertilized archegonia of CrLFY1_pro::GUS gametophytes (subsection “Spatial expression patterns of CrLFY1 are consistent with a retained ancestral role to facilitate cell divisions during embryogenesis”). In the case, the expression of CrLFY2 should to be important to function in the gametophytes. The spatial expression patterns of CrLFY2 would provide important information for the hypothesis. Since the authors succeeded both transformation and in situ hybridization in Ceratopteris, it seems technically possible to provide these data.

2) The authors provided that RNAi suppression analysis of CrLFY1 and CrLFY2 by using 4 different constructs and used wild type strain as control. The strain introduced with the vector is better to use as control. Transformation trials from three constructs produced a few transformants and the phenotype from same construct is different. The authors explained the difference of phenotype was because of the expression level of CrLFY1 or CrLFY2, though also should consider the effect of transformation procedure or transformation with the vector per se.

Reviewer #3:

This manuscript analyses the role of the LEAFY transcription factor in the fern Ceratopteris. The major finding is that LEAFY is expressed and essential for apical cell division both in the gametophyte and the sporophyte. LEAFY is a transcription factor playing a major role during flower development in angiosperms. It is present in most charophytes algae and all land plants. Its role has only been analyzed genetically in the moss Physcomitrella patens where it controls cell division in the sporophyte. In angiosperms, there is a growing body of evidence that LEAFY plays a role in meristem development (not necessarily flower meristem) as illustrated in various species such as rice, cucumber or pea. The evolutionary scenario describing how LEAFY could have been co-opted from a role in sporophyte cell division to flower development has remained very uncertain because of the lack of genetics in plant groups such as ferns and gymnosperms. In species belonging to these groups, one had to rely on expression patterns and inference on the function but there was, until now, no genetic evidence.

The work presented here represents a great leap forward by partially filling the gap between mosses and angiosperms. It analyses the expression and function of the LEAFY genes in Ceratopteris and concludes that LEAFY plays a role in apical growth in the sporophyte and gametophyte. To my opinion, these conclusions are well supported. Generating and analysing fern transgenics is a challenging but extremely useful task to learn about plant evolution in the major plant families. Even if the work is based mostly on expression pattern and mutant description (and it might look a bit old-fashioned at first glance for the lack of attempt to identify regulated genes), I am convinced that the results presented here are of great significance not only for the LEAFY research field but as an example of how a transcription factor can be co-opted along plant evolution to acquire a key role in angiosperms. Such a trajectory could not have been guessed without this type of seminal work in more basal plants.

My only concern is that this work is not so easy to follow for those who are not familiar with fern development. I suggest adding a scheme describing Ceraptopteris life cycle (and naming relevant organs and tissues) that can referred to as a guide for each figure showing expression patterns (GUS or mRNA) or phenotypes.