Minute clones, which lack a functional copy of the RpL14 gene, are eliminated via cell competition under axenic (A), normal (A’), and infected conditions (A’’). LacZ overexpression in loser cells is used as a negative control. Toll pathway inhibition via Cactus OE fails to rescue cell competition-driven elimination of minute clones under axenic conditions (B), whereas elimination is rescued under both normal (B’) and infected (B’’) conditions. Loser clones are highly apoptotic, as shown with a Dcp-1 staining (C, C’). and Cactus OE escaper clones under axenic conditions are extremely fragmented and apoptotic (Dcp-1 staining, (D, D’) Data are quantified by scoring the number of clones in the wing pouch (E), and by calculating the percentage of the normalized ratio between GFP+ and GFP- tissue areas in the wing pouch (F). Similarly, the elimination of loser clones with a single copy of dMyc in a background with two copies of dMyc is rescued specifically under normal or, even more efficiently, under infected conditions, but not under axenic conditions, as quantified in (G). ***p<0.001, **p<0.01, *p<0.05, n.s. not significant, Mann-Whitney test. Bars represent SEM.