(A) A schematic representation of dendrite pruning in ddaC neurons. (B–F) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 at WP and 16 hr APF. While the wild-type neurons …
Extended statistical data as Microsoft Excel spreadsheet.
(A–E) Live confocal images of da neurons expressing mCD8-GFP at w3L, WP, 16 hr and 20 hr, 32 hr APF. (A) While the control neurons pruned all the dendrites at 16 hr APF, ddaC neurons overexpressing p…
Extended statistical data as Microsoft Excel spreadsheet.
(A–E) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4, Gal44-77 or ppk-CD4-tdGFP at WP and 16 hr APF. While ddaC neurons overexpressing the UAS control construct pruned …
Extended statistical data as Microsoft Excel spreadsheet.
(A) Confocal images of ddaC neurons expressing mCD8-GFP and immunostained for anti-Patronin at w3L stage. ddaC somas are marked by dashed lines, and axons by arrows. Endogenous Patronin was detected …
Extended statistical data as Microsoft Excel spreadsheet.
(A–D, G–J) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 or Gal44-77 at WP and 16 hr APF. Low-level expressions of PatroninΔCH (B) and PatroninCKK (D), but not PatroninΔ…
Extended statistical data as Microsoft Excel spreadsheet.
(A) A schematic representation of full-length and truncated Patronin proteins and a summary of structure-function analysis of Patronin. Patronin contains multiple domains: a calponin homology (CH) …
Extended statistical data as Microsoft Excel spreadsheet.
(A–C, F–H) Confocal images of ddaC neurons expressing mCD8-GFP, Nod-β-gal or Kin-β-gal and immunostained for β-galactosidase at wL3 stage. ddaC somas are marked by asterisks, axons by arrows and …
Extended statistical data as Microsoft Excel spreadsheet.
(A) Confocal images of ddaC neurons expressing mCD8-GFP, Nod-β-gal and immunostained for β-galactosidase at wL3 stage. Nod-β-gal levels were reduced in the dendrites but enriched in the somas of patr…
(A–B, G–H) Representative kymographs of EB1-GFP comets in control or mutant ddaC dendrites. In control RNAi (A) or UAS control (G) ddaC neurons, dendritic EB1-GFP comets predominantly moved …
Extended statistical data as Microsoft Excel spreadsheet.
(A) Representative kymographs of EB1-GFP comets in control or mutant ddaC dendrites. In control RNAi ddaC neurons, dendritic EB1-GFP comets predominantly moved retrogradely towards the somas. …
Extended statistical data as Microsoft Excel spreadsheet.
(A, B) Representative kymographs of EB1-GFP comets in control or mutant ddaC dendrites. In patronin RNAi #1 and control RNAi co-expressing ddaC neurons (A), dendritic EB1-GFP comets moved …
Extended statistical data as Microsoft Excel spreadsheet.
(A) Quantification of the percentage of dendrite branches showing different levels of anterograde EB1-GFP comets in the control and mutant ddaC neurons. (B) Representative kymographs of EB1-GFP …
Extended statistical data as Microsoft Excel spreadsheet.
(A–C, F–H) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 at WP and 16 hr APF. Red arrowheads point to the ddaC somas. (A–C) ddaC neurons co-expressing patronin RNAi #1 …
Extended statistical data as Microsoft Excel spreadsheet.
(A–E) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 at 16 hr APF. Similar to the control neurons, klp10A RNAi (#1 or #2) or kat-60 RNAi (#1 or #2) ddaC neurons pruned …
Extended statistical data as Microsoft Excel spreadsheet.
(A–C) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 at WP and 16 hr APF. Red arrowheads point to the ddaC somas. ddaC neurons overexpressing the UAS control construct …
Extended statistical data as Microsoft Excel spreadsheet.
(A–B) Confocal images of ddaC neurons expressing mCD8-GFP, Nod-lacZ or Kin-lacZ and immunostained for β-galactosidase at wL3 stage. ddaC somas are marked by asterisks, axons by arrows and dendrites …
Extended statistical data as Microsoft Excel spreadsheet.
(A, D) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 at 16 hr APF. Low-level expression of PatroninCKK under the control of UASp promoter significantly rescued the …
Extended statistical data as Microsoft Excel spreadsheet.
(A–D) Live confocal images of ddaC neurons expressing mCD8-GFP driven by ppk-Gal4 at 16 hr APF. (A–B) While the control neurons pruned all the dendrites, cnnhk21 neurons (A), or APC2N175K, APC1Q8 …
Extended statistical data as Microsoft Excel spreadsheet.
Reagent type | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (D. melangoaster) | UAS-MicalN-ter | other | (Terman et al., 2002) | |
Genetic reagent (D. melangoaster) | SOP-flp (#42) | other | (Matsubara et al., 2011) | |
Genetic reagent (D. melangoaster) | ppk-Gal4 on II and III chromosome | other | (Grueber et al., 2003) | |
Genetic reagent (D. melangoaster) | UAS-Kin-β-gal | other | (Clark et al., 1997) | |
Genetic reagent (D. melangoaster) | UAS-EB1-GFP | other | (Stone et al., 2008) | |
Genetic reagent (D. melangoaster) | UASp-mCherry-Patronin | other | (Nashchekin et al., 2016) | |
Genetic reagent (D. melangoaster) | patroninc9-c5 | other | (Nashchekin et al., 2016) | |
Genetic reagent (D. melangoaster) | UAS-GFP-Patronin | other | (Derivery et al., 2015) | |
Genetic reagent (D. melangoaster) | UASp-Arf79F-EGFP | other | (Shao et al., 2010) | |
Genetic reagent (D. melangoaster) | UAS-Klp10A | this paper | ||
Genetic reagent (D. melangoaster) | UAS-GFP-Klp10A | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Patronin | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Venus-Patronin | this paper | ||
Genetic reagent (D. melangoaster) | UASp-PatroninΔCH | this paper | ||
Genetic reagent (D. melangoaster) | UASp-PatroninΔCKK | this paper | ||
Genetic reagent (D. melangoaster) | UASp-PatroninCKK | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Venus-PatroninΔCH | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Venus-PatroninΔCKK | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Venus-PatroninCC1-3 | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Venus-PatroninCKK | this paper | ||
Genetic reagent (D. melangoaster) | UAS-Venus-PatroninCH | this paper | ||
Genetic reagent (D. melangoaster) | Gal4109(2)80 | Bloomington Stock Center | BDSC: 8769 | |
Genetic reagent (D. melangoaster) | ppk-CD4-tdGFP | Bloomington Stock Center | BDSC: 35843 | |
Genetic reagent (D. melangoaster) | GSG2295-Gal4 | Bloomington Stock Center | BDSC: 40266 | |
Genetic reagent (D. melangoaster) | Gal44-77 | Bloomington Stock Center | BDSC: 8737 | |
Genetic reagent (D. melangoaster) | UAS-Nod-β-gal | Bloomington Stock Center | BDSC: 9912 | |
Genetic reagent (D. melangoaster) | patronin RNAi #2 | Bloomington Stock Center | BDSC: 36659 | |
Genetic reagent (D. melangoaster) | klp10A RNAi #2 | Bloomington Stock Center | BDSC: 33963 | |
Genetic reagent (D. melangoaster) | Gal42-21 | Bloomington Stock Center | FBal0328157 | |
Genetic reagent (D. melangoaster) | patronin RNAi # 1 | Vienna Drosophila RNAi Centre | VDRC: v108927 | |
Genetic reagent (D. melangoaster) | klp10A RNAi # 1 | Vienna Drosophila RNAi Centre | VDRC: v41534 | |
Genetic reagent (D. melangoaster) | control RNAi | Vienna Drosophila RNAi Centre | VDRC: v36355 | |
Genetic reagent (D. melangoaster) | control RNAi | Vienna Drosophila RNAi Centre | VDRC: v37288 | |
Genetic reagent (D. melangoaster) | patronink07433 | Drosophila Genetic Resource Center | #111217 | |
Genetic reagent (D. melangoaster) | patronin RNAi #3 | National Institute of Genetics, Japan | #18462 Ra-1 | |
Genetic reagent (D. melangoaster) | kat-60 RNAi # 1 | Vienna Drosophila RNAi Centre | VDRC: v38368 | |
Genetic reagent (D. melangoaster) | kat-60 RNAi # 2 | Vienna Drosophila RNAi Centre | VDRC: v106487 | |
Genetic reagent (D. melangoaster) | kat-60L1 RNAi # 1 | Vienna Drosophila RNAi Centre | VDRC: v31599 | |
Genetic reagent (D. melangoaster) | kat60-L1 RNAi # 2 | Vienna Drosophila RNAi Centre | VDRC: v108168 | |
Genetic reagent (D. melangoaster) | tau RNAi # 1 | Bloomington Stock Center | BDSC: 28891 | |
Genetic reagent (D. melangoaster) | tau RNAi # 2 | Bloomington Stock Center | BDSC: 40875 | |
Genetic reagent (D. melangoaster) | cnnhk21 | Bloomington Stock Center | BDSC: 5039 | |
Genetic reagent (D. melangoaster) | FRT 82B, APC2N175K, APC1Q8 | Bloomington Stock Center | BDSC: 7211 | |
Genetic reagent (D. melangoaster) | futschN94 | Bloomington Stock Center | BDSC: 8805 | |
Antibody | anti-β-galactosidase | Promega | Cat#: Z3783 | 1:1000 |
Antibody | anti-Patronin | M Gonzalez-Gaitan | 1:500 | |
Antibody | Cy3-conjugated goat anti-Rabbit antibody | Jackson | Cat#111-165-003 | 1:500 |
Antibody | 647-conjugated goat anti-Rabbit antibody | Jackson | Cat#111-605-144 | 1:500 |
Antibody | anti-Futsch | DSHB | 22c10 | 1:50 |
Supplementary list of fly strains.