The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane
- University of Washington, United States
Abstract
The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened Acinetobacter baumannii transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in E. coli. We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to Acinetobacter baumannii OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets.
Data availability
The cryo-EM map has been deposited in the Electron Microscopy Data Bank with accession code EMD-8738 (8.7 Å map). The coordinates for the MlaBDEF model have been deposited to PDB, accession code 6IC4.
Article and author information
Author details
Funding
National Institute of Allergy and Infectious Diseases (U19AI107775)
- Samuel I Miller
National Institutes of Health (R01GM118396)
- Justin M Kollman
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2019, Kamischke et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 5,404
- views
-
- 713
- downloads
-
- 89
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 89
- citations for umbrella DOI https://doi.org/10.7554/eLife.40171
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane
eLife 8:e40171.
https://doi.org/10.7554/eLife.40171
