The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane
- University of Washington, United States
- Cited 2
- Views 1,688
- Annotations
Abstract
The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened Acinetobacter baumannii transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in E. coli. We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to Acinetobacter baumannii OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets.
Article and author information
Author details
Funding
National Institute of Allergy and Infectious Diseases (U19AI107775)
- Samuel I Miller
National Institutes of Health (R01GM118396)
- Justin M Kollman
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Tâm Mignot, Aix Marseille University-CNRS UMR7283, France
Publication history
- Received: July 17, 2018
- Accepted: January 11, 2019
- Accepted Manuscript published: January 14, 2019 (version 1)
- Version of Record published: February 6, 2019 (version 2)
Copyright
© 2019, Kamischke et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 1,688
- Page views
-
- 303
- Downloads
-
- 2
- Citations
Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.
Download links
Downloads (link to download the article as PDF)
Download citations (links to download the citations from this article in formats compatible with various reference manager tools)
Open citations (links to open the citations from this article in various online reference manager services)
Further reading
-
- Microbiology and Infectious Disease
-
- Immunology and Inflammation
- Microbiology and Infectious Disease
