Ten countries have reported pfhrp2/pfhrp3 gene deletions since the first observation of pfhrp2-deleted parasites in 2012. In a previous study (Watson et al., 2017) we characterised the drivers selecting for pfhrp2/3 deletions, and mapped the regions in Africa with the greatest selection pressure. In February 2018, the World Health Organization issued guidance on investigating suspected false-negative rapid diagnostic tests (RDTs) due to pfhrp2/3 deletions. However, no guidance is provided regarding the timing of investigations. Failure to consider seasonal variation could cause premature decisions to switch to alternative RDTs. In response, we have extended our methods and predict that the prevalence of false-negative RDTs due to pfhrp2/3 deletions is highest when sampling from younger individuals during the beginning of the rainy season. We conclude by producing a map of the regions impacted by seasonal fluctuations in pfhrp2/3 deletions and a database identifying optimum sampling intervals to support malaria control programmes.
All data generated are provided within the online database, hosted through a shiny application at https://ojwatson.shinyapps.io/seasonal_hrp2/. The raw data for the application is available within the github repository at https://github.com/OJWatson/hrp2malaRia.
PfPR2-10 in Africa 2000-2015Malaria Atlas Project, http://www. map.ox.ac.uk/.
- Oliver John Watson
- Robert Verity
- Azra C Ghani
- Tini Garske
- Steven R Meshnick
- Jonathan B Parr
- Jonathan B Parr
- Hannah C Slater
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Ben Cooper, Mahidol Oxford Tropical Medicine Research Unit, Thailand
© 2019, Watson et al.
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