(A–E) Mean vacuole size of Toxoplasma gondii (Tg) dependent on different dosages of IFNγ pre-treatment for five different human cell lines converted to number of parasites per vacuole as per HRMAn decision tree machine learned algorithm. Plotted are the distribution of vacuoles that contain one parasite, two, four or more than four parasites. Data shown was recorded 24 hr post-infection. Growth restriction of type I (RH) Tg (left) or type II (Pru) Tg (right) in THP-1 cells (A), A549 cells (B), HeLa cells (C), HFF (D) and HUVECs (E). All data shown above represent the mean of N = 3 experiments±SEM. Significance was determined using non-parametric one-way Anova, n.s. = not significant, *p≤0.05; **p≤0.01, ***p≤0.001, ****p≤0.0001.