Negative reciprocity, not ordered assembly, underlies the interaction of Sox2 and Oct4 on DNA
- Harvard Medical School, United States
Figures
Figure 1
Ordered assembly, graphs and energy landscapes.
(A) Ordered assembly of Sox2 and Oct4 on DNA adapted from (Chen et al., 2014, Figure 6E) and annotated with the quantities described in the Introduction. (B) Corresponding linear framework graph for Sox2 and Oct4 binding to DNA, showing the microstates (vertices), transitions (directed edges) and rates (edge labels). (C) Hypothetical 2D energy landscape for the system in A, showing the free-energy minima corresponding to the four microstates. (D) Hypothetical 1D cross-section through the energy landscape, along a reaction coordinate that traverses the lowest point on the ‘continental divide’ between microstates o and so, as explained in the text. The annotations show the free-energy differences which influence the kinetic labels for binding of Sox2 to o () and for unbinding of Sox2 from so (). The thermodynamic ratio of the binding to unbinding label is determined by the difference in the free-energy minima (), as given by Equation 3.
Figure 2
The single-locus model and reciprocity.
(A) Linear framework graph for the TF , showing the microstate, b, in which is specifically bound to DNA and the microstate, nb, in which is not specifically bound. (B) Graph adapted from Figure 1B for the cell line in which Sox2 is measured and Oct4 is induced and taken to be at thermodynamic equilibrium, with only the ratios of binding to unbinding labels being shown. The subscripts and denote Sox2 and Oct4, respectively, the superscript denotes ‘induced’ and is the cooperativity. (C) As in B for the cell line in which Oct4 is measured and Sox2 is induced. Panels B and C define the single-locus model at thermodynamic equilibrium. (D) Plot of the reciprocity, , (orange surface), as defined in Equation 20, against the concentrations, [Sox2] and [Oct4], of the induced TFs, for the single-locus model with parameter values at thermodynamic equilibrium. The flat blue plane indicates . (E) As in panel D but for parameter values away from thermodynamic equilibrium, calculated as described in the text and the Materials and methods. Only a single off-rate, corresponding to from so to o in Figure 1B, was increased from the equilibrium value used in panel D, thereby breaking detailed balance. With this change, the reciprocity becomes negative throughout and can equal the reciprocity of calculated from the data of Chen et al. (Materials and methods), indicated by the flat brown plane. Numerical parameter values are given in the Materials and methods.
Figure 3
The genomic-diversity model and reciprocity.
(A) Schematic illustration of diversity in Sox2 and Oct4 binding sites on genomic DNA (thick gray curve), showing two types of loci, type 1 (black squares) and type 2 (blue squares), which have distinct ’s and ’s, as indicated by the subscripts, and , respectively. The details are shown for the cell line in which Sox2 is measured and Oct4 is induced, as in Figure 2B and a similar model should be imagined for the other cell line, as in Figure 2C. (B) Plot of the reciprocity, , as in Figure 2B, for the model in panel A at thermodynamic equilibrium with both types of loci having positive cooperativity. (C) As in panel B but with the off-rate corresponding to , for the transition from so to o in Figure 1B, increased at type II loci from the equilibrium value used in panel B, thereby breaking detailed balance. The flat brown plane marks the reciprocity, , of the data of Chen et al. (Materials and methods). (D) As in panels B and C but at thermodynamic equilibrium with mixed cooperativities, positive at type 1 loci and negative at type 2 loci. Numerical parameter values are given in the Materials and methods.
Scheme 1
Empirical distributions for (left) and (right).
https://doi.org/10.7554/eLife.41017.007Additional files
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Negative reciprocity, not ordered assembly, underlies the interaction of Sox2 and Oct4 on DNA
eLife 8:e41017.
https://doi.org/10.7554/eLife.41017
