1. Neuroscience

Two central pattern generators from the crab Cancer borealis respond robustly and differentially to extreme extracellular pH

  1. Jessica A Haley
  2. David Hampton
  3. Eve Marder  Is a corresponding author
  1. Brandeis University, United States
https://doi.org/10.7554/eLife.41877
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  1. Jessica A Haley
  2. David Hampton
  3. Eve Marder
(2018)
Two central pattern generators from the crab Cancer borealis respond robustly and differentially to extreme extracellular pH
eLife 7:e41877.
https://doi.org/10.7554/eLife.41877
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Abstract

The activity of neuronal circuits depends on the properties of the constituent neurons and their underlying synaptic and intrinsic currents. We describe the effects of extreme changes in extracellular pH - from pH 5.5 to 10.4 - on two central pattern generating networks, the stomatogastric and cardiac ganglia of the crab, Cancer borealis. Given that the physiological properties of ion channels are known to be sensitive to pH within the range tested, it is surprising that these rhythms generally remained robust from pH 6.1 to pH 8.8. The pH sensitivity of these rhythms was highly variable between animals and, unexpectedly, between ganglia. Animal-to-animal variability was likely a consequence of similar network performance arising from variable sets of underlying conductances. Together, these results illustrate the potential difficulty in generalizing the effects of environmental perturbation across circuits, even within the same animal.

Data availability

Raw .abf data files are available for download at https://doi.org/10.17605/osf.io/r7aes. All code is available for download at github.com/jesshaley/haley_hampton_marder_2019. Source data files have been provided for Figures 2, 3, 4, 6, 7, 8, and their figure supplements.

The following data sets were generated

Article and author information

Author details

  1. Jessica A Haley

    Volen Center, Brandeis University, Waltham, United States
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6282-7124

  2. David Hampton

    Volen Center, Brandeis University, Waltham, United States
    Competing interests
    No competing interests declared.

  3. Eve Marder

    Volen Center, Brandeis University, Waltham, United States
    For correspondence
    marder@brandeis.edu
    Competing interests
    Eve Marder, Deputy Editor, eLife.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-9632-5448

Funding

National Institutes of Health (R35 NS 097343)

  • Eve Marder

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Ronald L Calabrese, Emory University, United States

Version history

  1. Received: September 15, 2018
  2. Accepted: December 25, 2018
  3. Accepted Manuscript published: December 28, 2018 (version 1)
  4. Version of Record published: January 10, 2019 (version 2)

Copyright

© 2018, Haley et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Jessica A Haley
  2. David Hampton
  3. Eve Marder
(2018)
Two central pattern generators from the crab Cancer borealis respond robustly and differentially to extreme extracellular pH
eLife 7:e41877.
https://doi.org/10.7554/eLife.41877

Share this article

https://doi.org/10.7554/eLife.41877

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