(a) Superposition of the DEP domains of RGS7 (pink), RGS9 (green; PDB entry 2pbi), EPAC (yellow; PDB entry 2byv), pleckstrin (brown; PDB entry 1w4m), and Dvl (grey; PDB entry 1fsh) reveals diversity in the conformational organization of the β-hairpin arm. (b) Comparison of the DEP-DHEX domains of RGS7 and RGS9 shows differences in several loops and in the orientation of the DHEX domain. In RGS7, the DHEX domain is lifted upward thus generating a wide space between the domains. (c) Evaluation of the electrostatic surface potentials of the DEP-DHEX domains of RGS7 (left) and RGS9 (right). A strong basic patch (blue) is formed by three loops in the RGS7 structure but differences in the organization of these loops result in a more scattered distribution of basic charges in the RGS9 structure.