(A) Averaged traces of electrically evoked fluorescence from slices incubated with FFN, gold, or ACSF alone, blue. Traces reflect the average %ΔF/F, shading reflects ±SEM (n = 4 slices for ACSF, n = 6 slices for 10 μM FFN). (B) Box-and-whisker plot showing the AUC %ΔF/F evoked in the FFN and ACSF conditions. The edges of the boxes are 1 SEM from the mean, and the whiskers indicate ±2 SEM. Each point represents the average of images from one slice. ACSF slices were significantly different from FFN slices (two-tailed unpaired t-test, p < 0.01). For ACSF slices, the AUC %ΔF/F was 0.1 (CI95=[−0.78,0.98]) compared to 3.8 for FFN slices (CI95=[2.31,5.29]). (C) Transients from slices that were either incubated with FFN, gold, or FFN with 10 μM nomifensine, blue (n = 3 slices for FFN with nomifensine and n = 6 slices for FFN alone). (D) FFN102 transients evoked by electrical stimulation were strongly decreased by nomifensine (two-tailed unpaired t-test, p < 0.01; FFN, mean AUC %ΔF/F = 1.3 (CI95 = [0.61,1.99]) FFN with nomifensine, mean AUC %ΔF/F = 4.12 (CI95 = [2.97,5.27]).