Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs
- University of California, Berkeley, United States
- Memorial Sloan Kettering Cancer Center, United States
Abstract
Hypoosmotic conditions activate volume-regulated anion channels in vertebrate cells. These channels are formed by leucine-rich repeat-containing protein 8 (LRRC8) family members and contain LRRC8A in homo- or hetero-hexameric assemblies. Here we present single-particle cryo-electron microscopy structures of Mus musculus LRRC8A in complex with the inhibitor DCPIB reconstituted in lipid nanodiscs. DCPIB plugs the channel like a cork in a bottle - binding in the extracellular selectivity filter and sterically occluding ion conduction. Constricted and expanded structures reveal coupled dilation of cytoplasmic LRRs and the channel pore, suggesting a mechanism for channel gating by internal stimuli. Conformational and symmetry differences between LRRC8A structures determined in detergent micelles and lipid bilayers related to reorganization of intersubunit lipid binding sites demonstrate a critical role for the membrane in determining channel structure. These results provide insight into LRRC8 gating and inhibition and the role of lipids in the structure of an ionic-strength sensing ion channel.
Data availability
Final maps of LRRC8A-DCPIB in MSPE3D1 nanodiscs have been deposited to the Electron Microscopy Data Bank under accession codes EMDB-0562 (masked constricted state), and EMDB-0563 (masked expanded state) and atomic coordinates have been deposited in the PDB under IDs 6NZW (constricted state) and 6NZZ (expanded state). The original micrograph movies have been deposited to EMPIAR under accession codes EMPIAR-10366 and EMPIAR-10367. The map of apo-LRRC8A in MSP2N2 nanodiscs in a constricted state has been deposited with EMDB accession code EMDB-0564 and coordinates deposited in the PDB with ID 6O00.
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Final map of LRRC8A-DCPIB in MSPE3D1 nanodiscs (masked constricted state)Electron Microscopy Data Bank, EMDB-0562.
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Final map of LRRC8A-DCPIB in MSPE3D1 nanodiscs (masked expanded state)Electron Microscopy Data Bank, EMDB-0563.
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Atomic coordinates (LRRC8A-DCPIB in MSPE3D1 nanodiscs expanded state)Protein Data Bank, 6NZZ.
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Map of apo-LRRC8A in MSP2N2 nanodiscs in a constricted stateElectron Microscopy Data Bank, EMDB-0564.
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Coordinates of apo-LRRC8A in MSP2N2 nanodiscs in a constricted stateProtein Data Bank, 6O00.
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Original micrograph moviesElectron Microscopy Public Image Archive, EMPIAR-10366.
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Original micrograph moviesElectron Microscopy Public Image Archive, EMPIAR-10367.
Article and author information
Author details
Stephen Graf Brohawn
Funding
New York Stem Cell Foundation (NYSCF-R-N145)
- Stephen Graf Brohawn
National Institute of General Medical Sciences (DP2GM123496-01)
- Stephen Graf Brohawn
Klingenstein Third Generation Foundation (na)
- Stephen Graf Brohawn
McKnight Endowment Fund for Neuroscience (na)
- Stephen Graf Brohawn
National Cancer Institute (PO CA008748)
- Richard K Hite
Searle Scholars Program (na)
- Richard K Hite
Robertson Foundation (na)
- Richard K Hite
National Institute of General Medical Sciences (F32GM128263)
- David M Kern
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Kenton Jon Swartz, National Institute of Neurological Disorders and Stroke, National Institutes of Health, United States
Version history
- Received: October 7, 2018
- Accepted: February 14, 2019
- Accepted Manuscript published: February 18, 2019 (version 1)
- Version of Record published: February 28, 2019 (version 2)
- Version of Record updated: March 16, 2020 (version 3)
Copyright
© 2019, Kern et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs
eLife 8:e42636.
https://doi.org/10.7554/eLife.42636
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