A cross-modality enhancement of defensive flight via parvalbumin neurons in zona incerta
- University of Southern California, United States
Figures
Figure 1 with 2 supplements
Tactile stimulation enhances sound-induced flight response via SSp.
(A) Left, illustration of the head-fixed animal behavioral paradigm. Right, plots of running speed under noise presentation without (black) and with (red) concurrent whisker stimulation for an …
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Figure 1—source data 1
Data for Figure 1 and Figure 1—figure supplements 1 and 2.
- https://doi.org/10.7554/eLife.42728.005
Figure 1—figure supplement 1
Control experiments and analysis of individual animals.
(A) Illustration of the auditory and whisker stimulation paradigm. Right, photograph of the whisker stimulation detail. (B) Summary of baseline locomotion speed without (contr) and with (whisk) …
Figure 1—figure supplement 2
A flght test in freely moving mice.
(A) Illustration of the two-chamber flight test. (B) Summary of average speed for flight crossing the channel before and after whisker trimming. Data points for the same animal are connected with a …
Figure 2 with 1 supplement
The SSp-ZIv projection mediates the tactile enhancement of sound-induced flight.
(A) Illustration of the injection paradigm. (B) Anterogradely labeled axon terminals in rostral (left), dorsal and ventral (middle), as well as caudal (right) sectors of ZI. Scale bar, 200 µm. Blue …
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Figure 2—source data 1
Data for Figure 2 and Figure 2—figure supplement 1.
- https://doi.org/10.7554/eLife.42728.008
Figure 2—figure supplement 1
Analysis of individual animals and control experiment of LED stimulation alone.
(A) Normalized peak speed for each animal without (gray) and with (black) LED light stimulation. *p<0.05, two-sided unpaired t-test. Error bars represent mean ± s.e.m. (B) Normalized total travel …
Figure 3 with 3 supplements
PV+ neurons in ZIv mediate the tactile enhancement of flight behavior.
(A) Left, experimental paradigm. Right, Plots of speed without (black) and with (blue) LED stimulation for an example animal. Blue line marks the duration of noise/LED stimulation. (B) Summary of …
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Figure 3—source data 1
Data for Figure 3 and Figure 3—figure supplements 1–3.
- https://doi.org/10.7554/eLife.42728.013
Figure 3—figure supplement 1
Tests of efficacies of ChR2 and ArchT stimulation and analysis of individual animals.
(A) Confocal images showing the expression of ChR2 (green) and tdTomato (red) in ZIv in a PV-Cre::Ai14 animal. White arrows point to cells showing colocalization of ChR2 and tdTomato. Scale: 50 µm. …
Figure 3—figure supplement 2
Control experiments of LED stimulation alone.
(A, B) Summary of average locomotion speed without and with LED simulation alone for the ChR2 (A, n = 5 animals) and ArchT (B, n = 5 animals) group. ‘n.s.', non-significant, two-sided paired t-test. …
Figure 3—figure supplement 3
Test of efficacy of chemogenetic silencing.
(A) Current-clamp recording traces for an example hM4D(Gi)-expressing neuron in response to a series of current injections (500 ms duration) with amplitudes ranging from 0 to 120 pA with a step of …
Figure 4 with 2 supplements
The projection of ZIv PV+ neurons to POm enhances sound-induced flight.
(A) Illustration of injection paradigm. (B) Confocal images showing GFP expression at the injection site (left; scale: 500 µm) and in major target regions (middle and right; scale: 200 µm). Blue …
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Figure 4—source data 1
Data for Figure 4 and Figure 4—figure supplements 1 and 2.
- https://doi.org/10.7554/eLife.42728.017
Figure 4—figure supplement 1
Quantification of relative fluorescence density of GFP-labeled processes in different downstream regions of ZIv PV+ neurons (n = 4 animals).
Bar = s.d. Abbreviations: vlPAG, ventrolateral periaqueductal gray; dlPAG, dorsolateral periaqueductal gray; IC, inferior colliculus; MRN, midbrain reticular nucleus; RN, red nucleus; APN, anterior …
Figure 4—figure supplement 2
Analysis of individual animals and control experiment of LED stimulation alone.
(A) Normalized peak speed for each animal without (gray) and with (black) blue LED light stimulation. *p<0.05, two-sided unpaired t-test. Error bars represent mean ± s.e.m. (B), Normalized total …
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain (mouse) | Pvalb-ires-Cre | Jachson Laboratory | Stock No.008069 | |
| Strain (mouse) | Ai14 | Jachson Laboratory | Stock No.007914 | |
| Strain (mouse) | C57BL/6J | Jachson Laboratory | Stock No.000664 | |
| Recombinant DNA reagent | AAV2/1-CamKII-hChR2-eYFP-WPRE-hGh | UPenn Vector Core | ||
| Recombinant DNA reagent | AAV1-CAG-FLEX-eGFP-WPRE-bGH | UPenn Vector Core | ||
| Recombinant DNA reagent | AAV2/1-pEF1α-DIO-hChR2-eYFP | UPenn Vector Core | ||
| Recombinant DNA reagent | AAV1-CAG-FLEX-ArchT-GFP | UNC vector Core | ||
| Recombinant DNA reagent | pAAV-hSyn-hM4D(Gi)-mCherry | Addgene | Plasmid #50475 | |
| Other (stains) | NeuroTrace 640/660 Deep-Red Fluorescent Nissl Stain | ThermoFisher | N21483 | IHC 1:500 |
| Chemical compound, drug | Muscimol | ThermoFisher | M23400 | |
| Chemical compound, drug | Tetrodotoxin | Torcris | Cat. No.1078 | 1 µM |
| Chemical compound, drug | 4-Aminopyridine (4-AP) | Torcris | Cat. No. 0940 | 1 mM |
| Chemical compound, drug | cyanquixaline (CNQX) | Sigma-Aldrich | C239 | 20 µM |
| Chemical compound, drug | clozapine-N-oxide (CNO) | Torcris | Cat. No. 4936 | 1 mg/kg IP; 3 µM local infusion; 5 µM in slice recording |
| Software | Offline Sorter | Plexon | version 4 | |
| Software | MATLAB | Mathworks | version R2017a |
Additional files
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Supplementary file 1
Analysis of temporal profiles of speed traces in different sets of experiments.
Data are presented as mean ± SD. Two-sided paired t-test were performed to compared values between control and manipulation conditions. The type of experiment is shown by the corresponding figure number in main figures.
- https://doi.org/10.7554/eLife.42728.018
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Transparent reporting form
- https://doi.org/10.7554/eLife.42728.019
