Tomographic reconstruction of sample in Figure 5. The distribution of FAS particles is visible in the sequence of xy and xz slices and volume segmentation. Holey carbon film (light gray), FAS (orange), graphene contamination (dark grey), atmospheric ice contamination (dark blue) and vitreous buffer (cyan). Density map and orientations from subtomogram averaging were used to place copies of FAS into the tomographic reconstruction. Vertical section: single layer of particles adsorbed to the graphene-water interface.