Neuronal reactivation during post-learning sleep consolidates long-term memory in Drosophila

Table S1. LTM does not depend on the circadian time of training Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes according to Figure 1—figure supplement 1E, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S2. dopR1 mutant court mated females more than wild-type males Courtship indices (CIs) of naïve males of the indicated genotypes according to Figure 1—figure supplement 1G during 6 hr training with mated female are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that CIs of both groups are equal (H₀: CI_wt = CI_DopR1). Table S3. dopR1 mutant males do not learn Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes according to Figure 1—figure supplement 1H, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S4. Day-time sleep deprivation between 7–9 hr impairs LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes, sleep deprived as denoted in Figure 2A, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S5. Night-time sleep deprivation does not impair LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes, sleep deprived as denoted in Figure 2-figure supplement A, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S6. Silencing of DAN-aSP13 between 7–9 hr impairs LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes with DAN-aSP13 silenced as denoted in Figure 2B, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S7. Sleep induction between 5–7 hr consolidates STM to LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes and sleep induced as denoted in Figure 2C, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S8. DAN-aSP13 activation between 5–7 hr consolidates LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes with DAN-aSP13 activated as denoted in Figure 2D, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S9. Activation of vFB neurons between 5–7 hr consolidates LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes with vFB activated as denoted in Figure 5A, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c). Table S10. Silencing of vFB neurons between 7–10 hr impairs LTM Suppression indices (SIs) of naïve (train-) and experienced (train+) males of the indicated genotypes with vFB silenced as denoted in Figure 5B, tested in single-pair assays with mated females as trainers and testers. Courtship indices (CIs) are shown as median of n males and dispersion of the data as interquartile range (IQR). P values determined by permutation test for the null hypothesis that learning equals 0 (H₀: SI = 0) or for the null hypothesis that experimental and control males learn equally well (H₀: SI = SI_c).

Specific fly genotypes used in all main and supplementary figures.