Nanoscale organization of rotavirus replication machineries
- Universidad Nacional Autónoma de México, Mexico
- Universidad Autónoma del Estado de Morelos, Mexico
- Harvard Medical School, United States
Abstract
Rotavirus genome replication and assembly take place in cytoplasmic electron dense inclusions termed viroplasms (VPs). Previous conventional optical microscopy studies observing the intracellular distribution of rotavirus proteins and their organization in VPs have lacked molecular-scale spatial resolution, due to inherent spatial resolution constraints. In this work we employed super-resolution microscopy to reveal the nanometric-scale organization of VPs formed during rotavirus infection, and quantitatively describes the structural organization of seven viral proteins within and around the VPs. The observed viral components are spatially organized as 5 concentric layers, in which NSP5 localizes at the center of the VPs, surrounded by a layer of NSP2 and NSP4 proteins, followed by an intermediate zone comprised of the VP1, VP2, VP6. In the outermost zone, we observed a ring of VP4 and finally a layer of VP7. These findings show that rotavirus VPs are highly organized organelles.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Juan Manuel Rendon-Mancha
Funding
DGAPA-PAPIIT-UNAM (IG200317)
- Susana López
- Carlos F Arias
DGAPA-PAPIIT-UNAM (IA202417)
- Adán Guerrero
DGTIC-UNAM (SC15-1-IR-89)
- Adán Guerrero
Conacyt-Mexico (252213)
- Adán Guerrero
DGAPA-PAPIIT-UNAM (IN202312)
- Haydee Olinca Hernández
DGTIC-UNAM (SC16-1-IR-102)
- Adán Guerrero
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Arup K Chakraborty, Massachusetts Institute of Technology, United States
Version history
- Received: October 17, 2018
- Accepted: July 22, 2019
- Accepted Manuscript published: July 25, 2019 (version 1)
- Version of Record published: August 13, 2019 (version 2)
- Version of Record updated: August 22, 2019 (version 3)
- Version of Record updated: September 17, 2019 (version 4)
Copyright
© 2019, Garcés Suárez et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Nanoscale organization of rotavirus replication machineries
eLife 8:e42906.
https://doi.org/10.7554/eLife.42906
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