Parallel pathways for sound processing and functional connectivity among layer 5 and 6 auditory corticofugal neurons
- Massachusetts Eye and Ear Infirmary, United States
- Harvard Medical School, United States
Figures
Figure 1
Dual corticofugal pathways from L5 and L6.
(A) Illustration of transgenic and viral strategy used to selectively label L5 neurons that project to the inferior colliculus and MGB (left) or L6 neurons that project to the MGB (right). Cell body …
Figure 2 with 2 supplements
Antidromic phototagging of ACtx corticofugal projection neurons.
(A) Illustration of the strategy to activate neurons in ACtx L6 that project to the MGB (L6 CT). (B) Illustration of optogenetic approach to isolate L5 ACtx neurons with axons that innervate the IC …
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Figure 2—source data 1
Source data for Figure 2.
- https://doi.org/10.7554/eLife.42974.006
Figure 2—figure supplement 1
Classifying ACtx neuron identity.
(A) Four noise-evoked current source density plots (CSD) from recordings at different cortical depths. The CSD’s have been positioned to resemble the global CSD patterning of sinks and sources over …
Figure 2—figure supplement 2
Cell-type separation achieved using measured parameters
(A) A schematic showing the idealized separation between phototagged CF or CT neurons and their local neighborhoods (left). Bars represent an ideal d’ of ∞, since knowledge of the indivisible …
Figure 3 with 1 supplement
Sensory characterization of CF/CT projections.
(A) Example FRAs from a broadly-tuned L5 CF neuron (top) and a narrowly tuned L6 CT neuron (bottom). (B) Example PSTHs from a long-latency L5 CF neuron (top) and a short-latency L6 CT neuron (bottom)…
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Figure 3—source data 3
Source data for Figure 3.
- https://doi.org/10.7554/eLife.42974.009
Figure 3—figure supplement 1
Correlations between sensory tuning parameters and evoked firing rates.
(A) Significant correlation between sparsity and bandwidth parameters. Dotted line represents a linear fit to the data. (B) No significant correlation between latency and bandwidth. (C) No …
Figure 4
Predicting cell-type and layer from neural tuning parameters.
(A) Scatter plot of tuning parameters for both CF/CT cell-types, with the optimal SVM hyperplane. (B) Scatter plot of tuning parameters for L5/L6 cell-types, with the optimal SVM hyperplane. (C) …
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Figure 4—source data 1
Source data for Figure 4.
- https://doi.org/10.7554/eLife.42974.011
Figure 5 with 1 supplement
Modeling the stimulus-response function of L5 and L6 neurons.
(A) Snippets of DRC-evoked neural activity with corresponding model predictions for an example L5 CF (top) and L6 CT neuron (bottom). Predictive powers for the model fits are shown on the right. (B) …
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Figure 5—source data 1
Source data for Figure 5.
- https://doi.org/10.7554/eLife.42974.014
Figure 5—figure supplement 1
Further quantification of stimulus-evoked variability, model evaluation, and CGF structure.
(A) Scatter plot of signal and noise powers for CF/CT neurons. Histograms show the marginal distributions along the corresponding axes. Filled/non-filled bars represent neurons that had signal …
Figure 6
Feedforward connectivity within deep-layer cortical networks.
(A) Schematic of the 32-channel recording probe alongside PSTHs corresponding to each recording site. A 1 ms laser pulse (vertical blue lines) to the axon terminals for L5 CF (left) and L6 CT …
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Figure 6—source data 1
Source data for Figure 6.
- https://doi.org/10.7554/eLife.42974.016
Figure 7
Temporal interactions within deep-layer cortical networks.
(A) Schematic illustrating the four recorded cell-types and the cross-covariance approach. (B) An example cross-covariance function highlighting the temporal interaction between a L6 CT↔FS pair. (C–D…
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Figure 7—source data 1
Source data for Figure 7.
- https://doi.org/10.7554/eLife.42974.018
Author response image 1
Additional files
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Supplementary file 1
Supplemental tables detailing sample sizes, parameter values, and firing rates.
Supplemental Table S1. Summary table detailing sample sizes and parameter values for all sensory characterization comparisons (Figures 3–5). Supplemental Table S2. Summary table detailing sample sizes for all connectivity comparisons (Figures 6–7). Supplemental Table S3. Summary table detailing firing rates evoked by all presented stimuli.
- https://doi.org/10.7554/eLife.42974.019
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Transparent reporting form
- https://doi.org/10.7554/eLife.42974.020
