(A–B’) Cross-sections of the ciliary body from a control mouse (A, A’) and from a mouse with mosaic EC-specific deletion of Ctnnb1 exon 3 (B, B’), immunostained as indicated. GLUT1 is expressed in epithelial cells surrounding the ciliary body capillaries. ECs in the control ciliary body are CLDN5-/PLVAP +and ECs with deletion of Ctnnb1 exon 3 are CLDN5+/PLVAP- (arrows in A’ and B’). Inset in (A), schematic of the mouse eye showing the locations of the ciliary body, lens, and retina. Scale bar, 100 μm. (C–D”) Cross-sections of retina from a control mouse (C, C’, C”) and from a mouse with mosaic EC-specific deletion of Ctnnb1 exon 3 (D, D, D”), immunostained as indicated. With EC-specific deletion of Ctnnb1 exon 3, choriocapillaris ECs switch from CLDN5-/PLVAP +to CLDN5+/PLVAP-. The adjacent RPE is GLUT1+. Intra-retinal ECs remain CLDN5+/PLVAP-. (C”) and (D”) show higher magnification views of the boxed regions from panels (C’) and (D’). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium; CC, choriocapillaris. Scale bar for C-D’, 100 um. Scale bar for C’ and D’, 20 μm. (E,F) Lower magnification images encompassing the regions shown in (C’) and (D’). Scale bar, 200 um. (G,H) Flat mount of the retinal pigment epithelium, choroid, and sclera after the retina was removed, viewed from the anterior of the eye. With EC-specific deletion of Ctnnb1 exon 3, capillary ECs in the choriocapillaris switch from CLDN5-/PLVAP +to CLDN5+/PLVAP-. The ciliary body shows less complete conversion. Scale bar, 200 μm.