Cold Inducible RNA-binding protein (CIRBP) adjusts clock-gene expression and REM-sleep recovery after sleep deprivation
- University of Lausanne, Switzerland
Abstract
Sleep depriving mice affects clock-gene expression, suggesting that these genes contribute to sleep homeostasis. The mechanisms linking extended wakefulness to clock-gene expression are, however, not well understood. We propose CIRBP to play a role because its rhythmic expression is i) sleep-wake driven and ii) necessary for high-amplitude clock-gene expression in vitro. We therefore expect Cirbp knock-out (KO) mice to exhibit attenuated sleep-deprivation induced changes in clock-gene expression, and consequently to differ in their sleep homeostatic regulation. Lack of CIRBP indeed blunted the sleep-deprivation incurred changes in cortical expression of Nr1d1 whereas it amplified the changes in Per2 and Clock. Concerning sleep homeostasis, KO mice accrued only half the extra REM sleep wild-type (WT) littermates obtained during recovery. Unexpectedly, KO mice were more active during lights-off which was accompanied with faster theta oscillations compared to WT mice. Thus, CIRBP adjusts cortical clock-gene expression after sleep deprivation and expedites REM-sleep recovery.
Data availability
Source data files underlying all figures have been provided.
Article and author information
Author details
Marieke MB Hoekstra
Funding
Swiss National Science Foundation (146694)
- Marieke MB Hoekstra
Etat de Vaud
- Marieke MB Hoekstra
- Yann Emmenegger
- Jeffrey Hubbard
- Paul Franken
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Louis J Ptáček, University of California, San Francisco, United States
Ethics
Animal experimentation: All experiments were approved by the Ethical Committee of the State of Vaud Veterinary Office Switzerland under license VD2743 and 3201.
Version history
- Received: November 6, 2018
- Accepted: February 4, 2019
- Accepted Manuscript published: February 5, 2019 (version 1)
- Version of Record published: February 18, 2019 (version 2)
- Version of Record updated: February 25, 2019 (version 3)
Copyright
© 2019, Hoekstra et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 3,174
- Page views
-
- 400
- Downloads
-
- 38
- Citations
Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Cold Inducible RNA-binding protein (CIRBP) adjusts clock-gene expression and REM-sleep recovery after sleep deprivation
eLife 8:e43400.
https://doi.org/10.7554/eLife.43400
