Stiffness and tension gradients of the hair cell's tip-link complex in the mammalian cochlea
- PSL Research University, France
- Sorbonne Université, France
Abstract
Sound analysis by the cochlea relies on frequency tuning of mechanosensory hair cells along a tonotopic axis. To clarify the underlying biophysical mechanism, we have investigated the micromechanical properties of the hair cell's mechanoreceptive hair bundle within the apical half of the rat cochlea. We studied both inner and outer hair cells, which send nervous signals to the brain and amplify cochlear vibrations, respectively. We find that tonotopy is associated with gradients of stiffness and resting mechanical tension, with steeper gradients for outer hair cells, emphasizing the division of labor between the two hair-cell types. We demonstrate that tension in the tip links that convey force to the mechano-electrical transduction channels increases at reduced Ca2+. Finally, we reveal gradients in stiffness and tension at the level of a single tip link. We conclude that mechanical gradients of the tip-link complex may help specify the characteristic frequency of the hair cell.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 2, 3, 5 and 6.
Article and author information
Author details
Nicolas Antoine Michalski
Funding
French National Agency for Research (ANR-11-BSV5-011)
- Pascal Martin
Labex Celltisphybio part of the Idex PSL (ANR-10-LABX-0038)
- Pascal Martin
French National Agency for Research (ANR-16-CE13-0015)
- Pascal Martin
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Doris K Wu, National Institutes of Health, United States
Ethics
Animal experimentation: All experimental procedures were approved by the Ethics committee on animal experimentation of the Institut Curie; they complied with the European and French-National Regulation for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Directive 2010/63; French Decree 2013-118).
Version history
- Received: November 7, 2018
- Accepted: March 27, 2019
- Accepted Manuscript published: April 1, 2019 (version 1)
- Version of Record published: April 15, 2019 (version 2)
Copyright
© 2019, Tobin et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Stiffness and tension gradients of the hair cell's tip-link complex in the mammalian cochlea
eLife 8:e43473.
https://doi.org/10.7554/eLife.43473
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