(A) Comparison between evoked slow events and spontaneous occurring slow events. Top two traces are pre- and post-junctional events in pair recording aligned by the time of peak response. Middle, spontaneous slow events recorded from the same cell. Dark lines represent the average responses. Lower traces, the two average responses normalized and superimposed. (B) Top, superimposed traces of light evoked chemical synaptic potentials recorded in an olivary neuron from Thy1 mouse. Middle, superimposed traces of spontaneous spikelets from the same neuron. Dark lines represent the average responses, black bar is the duration of light stimulation. Lower traces, the two average responses normalized and superimposed. (C) Scatter plot of rise time and amplitude (top) and rise time and duration at half amplitude (bottom) of slow events (colored close circles) and chemical synaptic events (open circles). Each point represents an average response in one cell (n = 17 cells). Matching color denotes the same cell. Evoked slow events from different neurons are plotted as black circles (n = 30 cells). (D) Example of evoked slow events in paired recording before (gray) and after (orange) addition of DNQX. (E) The distribution of the average amplitude, duration and rise time of spontaneous slow events before (gray) and after (orange) addition of DNQX and APV (n = 9 cells).