(A) H1-HeLa cells were incubated with 100 µg/ml α-sarcin or 50 µg/ml Restrictocin A for 6 hr at 37˚C, followed by treatment with cycloheximide for 30 min prior to a 20 min pulse with 20 µg/ml puromycin. Protein lysates were harvested, resolved by SDS-PAGE and subjected to immunoblotting using antibodies against puromycin and actin. (B) H1-HeLa cells were adsorbed for 1 hr with gradient-purified eHAV or naked HAV (~1000 GEs/cell) at 37˚C or with HRV14 (10 PFU/cell) at 33˚C in presence or absence of 50 µg/ml Restrictocin A. The inoculum was removed, cells were rinsed with PBS, and incubated for another 5 hr. Cells were then pulsed with puromycin for 20 min and protein lysates were harvested, resolved by SDS-PAGE, and subjected to immublobotting using antibodies against puromycin and actin. Band intensities were quantified, normalized to loading control, and presented as a percentage of virus plus α-sarcin relative to virus alone. Alternatively, viruses were incubated overnight at 4˚C with human ‘JC’ plasma containing neutralizing HAV antibodies and inoculated as such.