Need-based prioritization of behavior
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, United States
- National Institute on Drug Abuse (NIDA), National Institutes of Health, United States
- Brown University Graduate Partnerships Program, United States
- Brown University, United States
Figures
Figure 1 with 1 supplement
Prolonged food-deprivation leads to more pronounced changes in rodent physiology.
(A–F) 18 hr food restriction reduced body weight (A), fat mass (B), lean mass (C), and tissue weights of inguinal WAT, epididymal WAT, quadriceps, gastrocnemius, liver and stomach (D) in both males …
Figure 1—figure supplement 1
Prolonged food-deprivation leads to more pronounced changes in rodent physiology.
(A) Schematic of appetite states used for evaluations. (B) Overall body weight changes of animals at the start of the experiment compared to the time of dissection. Food restriction markedly lowered …
Figure 2 with 3 supplements
Evaluating resident behavior in response to a receptive female intruder.
(A) Catalogue of scored resident behaviors (top); mounting and/or intromission of the female intruder (red), pursuit and/or attempted mounting of the female intruder (yellow), anogenital …
Figure 2—figure supplement 1
Experimental design of resident-intruder assay during evaluation of resident behavior.
(A) Brain schematic of optogenetic surgery whereby Agrp-IRES-Cre animals were unilaterally injected with Cre-dependent ChR2-tdTomato virus and unilaterally implanted with an optical fiber over the …
Figure 2—figure supplement 2
Evaluating resident behavior in response to a receptive female intruder.
(A) Raster plots of second-to-second scored behaviors of all resident animals across the entire Trial Phase (B–F) Quantified within-subject comparisons of resident food intake in the absence or …
Figure 2—figure supplement 3
Post-trial phase food intake in resident mice in female intruder paradigm.
(A) Quantified between-subject comparisons of resident Post-phase food intake in animals never exposed to a female intruder or previously exposed to a female intruder during the Trial-phase. (B–F) …
Figure 3
Behavioral sequence analyses of resident males exposed to a female intruder.
(A–B) Transition matrices of behavioral transitions grouped by appetite condition in the presence (A) or absence (B) of food. (C) Quantified between-subject comparisons of the cosine distance from …
Figure 4 with 2 supplements
Evaluating resident behavior in response to a subordinate male intruder.
(A) Catalogue of scored resident behaviors (top); aggressive bouts of attack (red), chasing (yellow), anogenital chemoinvestigation (orange), nose-to-nose chemoinvestigation (green), drinking …
Figure 4—figure supplement 1
Evaluating resident behavior in response to a subordinate male intruder.
(A) Raster plots of second-to-second scored behaviors of all resident animals across the entire Trial Phase (B–F) Quantified within-subject comparisons of resident food intake in the absence or …
Figure 4—figure supplement 2
Post-trial phase food intake in resident mice in male intruder paradigm.
(A) Quantified between-subject comparisons of resident Post-phase food intake in animals never exposed to a male intruder or previously exposed to a male intruder during the Trial-phase. (B–F) …
Figure 5 with 2 supplements
Evaluating female intruder behavior in response to a dominant male resident.
(A) Catalogue of scored female intruder behaviors (top); receptivity, encompassing presentation/lordosis (red), non-receptivity, comprising of escape/attempted escape (yellow), anogenital …
Figure 5—figure supplement 1
Experimental design of resident-intruder assay during evaluation of intruder behavior.
(A) Brain schematic of optogenetic surgery whereby Agrp-IRES-Cre animals were unilaterally injected with Cre-dependent ChR2-tdTomato virus and unilaterally implanted with an optical fiber over the …
Figure 5—figure supplement 2
Evaluating female intruder behavior in response to a dominant male resident.
(A–J) Quantified within-subject comparisons of female intruder food intake in the intruder homecage versus empty resident cage versus occupied resident cage (A,C,E,G,I) and cumulative latency plots …
Figure 6
Behavioral sequence analyses of resident males exposed to a male intruder.
(A–B) Transition matrices of behavioral transitions grouped by appetite condition in the presence (A) or absence (B) of food. (C) Quantified between-subject comparisons of the cosine distance from …
Figure 7 with 1 supplement
Evaluating male intruder behavior in response to a dominant male resident.
(A) Catalogue of scored male intruder behaviors (top); defensive posturing such as rearing and being pinned (red), flight comprising of escape/jumping (yellow), anogenital chemoinvestigation …
Figure 7—figure supplement 1
Evaluating male intruder behavior in response to a dominant male resident.
(A–J) Quantified within-subject comparisons of male intruder food intake in the intruder homecage versus empty resident cage versus occupied resident cage (A,C,E,G,I) and cumulative latency plots to …
Figure 8
Summary Model: Feeding and social interactions/cues dynamically compete for behavioral expression.
(A) When resident male mice are presented with a singular external incentive (food), they express feeding based on internal state (hunger intensity). (B) When resident male mice are presented with …
Additional files
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Source code 1
Custom python scripts.
- https://doi.org/10.7554/eLife.44527.019
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Source code 2
Custom MATLAB script.
- https://doi.org/10.7554/eLife.44527.020
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Transparent reporting form
- https://doi.org/10.7554/eLife.44527.026
