(A) Top: Z-section of the double transgenic line Tg(kdrl:nls-mCherry; myl7:GFP) showing the overall structure of the heart. Bottom: Scheme of the zebrafish heart with the endocardium, myocardium and …
(A) Experimental set-up for the photoconversion studies. Heart was stopped at 72 hpf, the region of interest exposed to 405 nm light to convert kaede from green to red (shown in magenta) fluorescent …
(A) Z-sections of the Tg(fli:gal4FF/UAS:Kaede) line just after photoconversion (72 hpf), and at 96 hpf and 120 hpf. The star highlights the photoconverted cell in the top, middle and bottom part of …
(A) Staining of Fibronectin1 (magenta) on Tg(myl7:GFP; fli1a:nls-mCherry), highlighting the myocardium (white) and the endothelium (green) at 72 hpf. Scale bar: 20 μm. V: ventricle. Fibronectin1 …
(A) Z-sections of the Tg(kdrl:nls-mCherry) line counterstained by DAF-FMDA (cyan) and Fn1 (magenta) at 72 hpf, 96 hpf and 120 hpf. Scale bar: 20 µm. (B) Co-localization of Fibronectin1 (magenta) …
(A) and Tg(tp1:dGFP) (B) at 72hpf. OFT: outflow tract, At: atrium, V: ventricle. Scale bar: 20 µm. (C) Confocal z-section of OFT valves expressing the Klf2a reporter and Notch reporter at 72 hpf, 96 …
Fluorescence intensity measurements.
Scale bar: 20 µm. Results obtained from three independent experiments.
(A) Confocal z-sections of the double transgenic line 4xGTIIC:d2GFP; kdrl:membrane-mCherry counterstained with the Elnb antibody and focused on the OFT. Scale bar: 20 µm. (B) Example of the valve …
Phenotypic quantifications.
Asterisks show endothelial cells expressing Yap1. At: atrium, V: ventricle. Scale bar: 20 µm. V: ventricle, At: atrium. Resultsobtained from three independent experiments.
Quantification of the Klf2a and Notch reporter expressions in tnnt2a-morpholino injected embryos showing a ‘beating heart’ (p=0,27 and p=0,01 respectively). (A) and a ‘slow beating heart’ (p=0,7 and …
Phenotypic and fluorescence reporters quantifications.
Quantification of the valve phenotypes at 72 hpf, 96 hpf and 120 hpf (normal, thick, delayed) in klf2a+/+(n = 11), and klf2a-/- (n = 12) using the Tg(fli:gal4FF/UAS:Kaede). (A) and notch1b+/+ (n = 7)…
Fluorescent intensity quantifications.
(A) In situ hybridization of notch1b in wild-type embryos at 48 hpf and 72 hpf. In situ hybridization of notch1b in klf2a+/+ (n = 134 and n = 107 at 48 hpf and 72 hpf respectively) and klf2a-/- (n = …
(A) Z-sections and quantifications of the valves phenotypes (normal, thick, delayed) of the Tg(fli:lifeaAct-EGFP; kdrl:nls-mCherry) at 72 hpf (B), 96 hpf (C) and 120 hpf (D) in trpp2+/+(n = 11, n = 1…
Phenotypic and fluorescence reporters quantifications.
(A) Relative fractional shortening in the atrium and ventricle in control, vlad tepes mutant, trpp2-/-, trpp2-/-; trpv4-/-, piezo1-/-, piezo1-/-; piezo2a-/-. (B) Flow profile in the OFT at 72 hpf …
(A) Z-sections of the Tg(fli:lifeAct-EGFP: kdrl:nls-mCherry) in control (trpp2+/+ and trpp2+/-) and trpp2-/- obtained from three independent experiments and in control (piezo1+/+) and piezo1-/- …
(A) Z-projection of the RNAscope of kdrl and single Z-sections of the RNAscope of trpp2 and piezo1 on 72 hpf wild-type embryos in the head and the OFT. (B) Z-section of the Tg(piezo1:nls-Venus) …
Two cell layers forming the OFT respond to piezo1 activity: the endothelium (green) and smooth muscle cells (magenta). Endothelial klf2a expression is repressed by Piezo1. In the smooth muscle …