(A) Silencing Integrin-β1 in the liver results in distortion of both bile canalicular and sinusoidal networks, with reduced apparent alignment with the CV-PV axis in comparison to control conditions. Shown are representative samples for control conditions -upper panels, siRNA against Luciferase (Luc) and Integrin-β1 knock down -lower panels, siRNA against Integrin-β1 receptor (KD) stained for bile canalicular network (left, CD13 staining), sinusoidal network (middle, fibronectin/laminin staining), and merge (right). All panels correspond to maximal intensity z-projection of 60 μm of liver tissue. (B) Individual hepatocytes retain their biaxial cell polarity in Integrin-β1 KD, as revealed by cross-correlation analysis of nematic cell polarity axes for apical and basal plasma membrane patterns (analogous to Figure 2F). (C, D) In contrast, the alignment of biaxial cell polarity axes and the local preferred direction of the sinusoidal network with are reduced in Integrin-β1 KD. Panel C shows bipolar cell polarity axes of apical plasma membrane color-coded according to their alignment with the local reference direction () (analogous to Figure 3D). Panel D shows the quantification of alignment of apical bipolar axis, apical ring axis, and preferred sinusoid orientation with local reference direction (analogous to Figure 3G). A detailed graphical representation can be found in Figure 4—figure supplement 1. Statistics in B, D: mean+/-s.d. for n=5 animals (Luc) and n=4 animals (KD); statistical significance: panel B: , , , ; panel D: , , , two-sided t-test assuming unequal variances).