Self-capping of nucleoprotein filaments protects Newcastle Disease Virus genome

Abstract
Data availability
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Abstract

Non-segmented negative-strand RNA viruses, such as Measles, Ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode, and the tightly packed structure functions as a seed for nucleocapsid to assemble from both directions and grows into double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations on its loop interface yielded a single-headed unfunctional filament.

Data availability

The cryo-EM density map has been deposited in EMDB with the accession number EMD-9793. The atom coordinates of the structure have been deposited in PDB with the PDB ID 6JC3.

The following data sets were generated

1. Xiyong S
2. Hong S
3. Yanping Z
4. Wei D
5. Songying O
6. Qing-Tao S
7. Zhi-Jie
8. L
(2019) The Cryo-EM structure of nucleoprotein-RNA complex of Newcastle disease virus
RCSB Protein Data Bank, 6JC3.

http://www.rcsb.org/pdb/6JC3
1. Xiyong S
2. Hong S
3. Yanping Z
4. Wei D
5. Songying O
6. Qing-Tao S
7. Zhi-Jie
8. L
(2019) The Cryo-EM structure of nucleoprotein-RNA complex of Newcastle disease virus
Electron Microscopy Data Bank, EMD-9793.

http://www.ebi.ac.uk/pdbe/entry/emdb/EMD-9793

The following previously published data sets were used

1. Alayyoubi M
2. Leser GP
3. Kors CA
4. Lamb RA
(2015) Structure of the parainfluenza virus 5 nucleocapsid-RNA complex: an insight into paramyxovirus polymerase activity
PDB: 4XJN.

http://www.rcsb.org/structure/4XJN
1. Cai S
2. Li J
3. Wong MT
4. Jiao P
5. Fan H
6. Liu D
7. Liao M
8. Jiang J
9. Shi M
10. Lam TT
11. Ren T
12. Leung FC
(2011) Genetic characterization and evolutionary analysis of 4 Newcastle disease virus isolate full genomes from waterbirds in South China during 2003-2007
GenBank: HM063424.1.

https://www.ncbi.nlm.nih.gov/nuccore/HM063424.1

Article and author information

Author details

Xiyong Song

Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China

Competing interests
The authors declare that no competing interests exist.
Hong Shan

iHuman Institute, ShanghaiTech University, Shanghai, China

Competing interests
The authors declare that no competing interests exist.
Yanping Zhu

National Laboratory of Biomacromolecules, Chinese Academy of Sciences, Beijing, China

Competing interests
The authors declare that no competing interests exist.
Shunlin Hu

College of Veterinary Medicine, Yangzhou University, Yangzhou, China

Competing interests
The authors declare that no competing interests exist.
Ling Xue

College of Veterinary Medicine, Yangzhou University, Yangzhou, China

Competing interests
The authors declare that no competing interests exist.
Yong Chen

National Laboratory of Biomacromolecules, Chinese Academy of Sciences, Beijing, China

Competing interests
The authors declare that no competing interests exist.
Wei Ding

Center for Biological Imaging, Chinese Academy of Sciences, Beijing, China

Competing interests
The authors declare that no competing interests exist.
Tongxin Niu

Center for Biological Imaging, Chinese Academy of Sciences, Beijing, China

Competing interests
The authors declare that no competing interests exist.
Jian Gu

College of Veterinary Medicine, Yangzhou University, Yangzhou, China

Competing interests
The authors declare that no competing interests exist.
Songying Ouyang

College of Life Sciences, Fujian Normal University, Fuzhou, China

For correspondence
ouyangsy@fjnu.edu.cn

Competing interests
The authors declare that no competing interests exist.
Qing-Tao Shen

iHuman Institute, ShanghaiTech University, Shanghai, China

For correspondence
shenqt@shanghaitech.edu.cn

Competing interests
The authors declare that no competing interests exist.
Zhi-Jie Liu

Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China

For correspondence
liuzhj@shanghaiTech.edu.cn

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-7279-2893

Funding

National Nature Science Foundation of China grant (31330019)

Zhi-Jie Liu

National Nature Science Foundation of China grant (31770948)

Songying Ouyang

National Nature Science Foundation of China grant (31570875)

Songying Ouyang

National Natural Science Foundation of China grant (81590761)

Songying Ouyang

the National Key R&D program of China (2017YFA0504800)

Qing-Tao Shen

Yunnan Provincial Science and Technology Department Project (2016FC007)

Zhi-Jie Liu

The Pujiang Talent program (17PJ1406700)

Qing-Tao Shen

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.