One bout of neonatal inflammation impairs adult respiratory motor plasticity in male and female rats
- University of Oregon, United States
- University of Wisconsin, United States
Abstract
Neonatal inflammation is common and has lasting consequences for adult health. We investigated the lasting effects of a single bout of neonatal inflammation on adult respiratory control in the form of respiratory motor plasticity induced by acute intermittent hypoxia, which likely compensates and stabilizes breathing during injury or disease and has significant therapeutic potential. Lipopolysaccharide-induced inflammation at postnatal day four induced lasting impairments in two distinct pathways to adult respiratory plasticity in male and female rats. Despite a lack of adult pro-inflammatory gene expression or alterations in glial morphology, one mechanistic pathway to plasticity was restored by acute, adult anti-inflammatory treatment, suggesting ongoing inflammatory signaling after neonatal inflammation. An alternative pathway to plasticity was not restored by anti-inflammatory treatment, but was evoked by exogenous adenosine receptor agonism, suggesting upstream impairment, likely astrocytic-dependent. Thus, the respiratory control network is vulnerable to early-life inflammation, limiting respiratory compensation to adult disease or injury.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files. Source data are available for all figures.
Article and author information
Author details
Adrianne G Huxtable
Funding
National Institutes of Health (HL141249)
- Adrianne G Huxtable
National Institutes of Health (HL111598)
- Jyoti J Watters
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee protocols (#18-02) of the University of Oregon. All surgeries were performed under isoflurane or urethane anesthesia and every effort was made to minimize pain, distress, or discomfort.
Reviewing Editor
- Jan-Marino Ramirez, Seattle Children's Research Institute and University of Washington, United States
Publication history
- Received: January 22, 2019
- Accepted: March 21, 2019
- Accepted Manuscript published: March 22, 2019 (version 1)
- Version of Record published: April 15, 2019 (version 2)
Copyright
© 2019, Hocker et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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One bout of neonatal inflammation impairs adult respiratory motor plasticity in male and female rats
eLife 8:e45399.
https://doi.org/10.7554/eLife.45399
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