Flavodiiron proteins 1-to-4 function in versatile combinations in O2 photoreduction in cyanobacteria
Abstract
Flavodiiron proteins (FDPs) constitute a group of modular enzymes widespread in all life Domains. Synechocystis sp. PCC 6803 has four FDPs (Flv1-4), which are essential for the photoprotection of photosynthesis. A direct comparison of light-induced O2 reduction (Mehler-like reaction) under high (3% CO2, HC) and low (air level CO2, LC) inorganic carbon conditions demonstrated that the Flv1/Flv3 heterodimer is solely responsible for an efficient steady-state O2 photoreduction under HC, with flv2 and flv4 expression strongly down-regulated. Conversely, under LC conditions, Flv1/Flv3 acts only as a transient electron sink, due to the competing withdrawal of electrons by the highly induced NDH-1 complex. Further, in vivo evidence is provided indicating that Flv2/Flv4 contributes to the Mehler-like reaction when naturally expressed under LC conditions, or, when artificially overexpressed under HC. The O2 photoreduction driven by Flv2/Flv4 occurs down-stream of PSI in a coordinated manner with Flv1/Flv3 and supports slow and steady-state O2 photoreduction.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
Suomen Akatemia (315119)
- Yagut Allahverdiyeva-Rinne
NordForsk (82845)
- Eva-Mari Aro
- Yagut Allahverdiyeva-Rinne
Koneen Säätiö (7fa491)
- Yagut Allahverdiyeva-Rinne
Suomen Akatemia (307335)
- Eva-Mari Aro
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Jürgen Kleine-Vehn, University of Natural Resources and Life Sciences, Austria
Version history
- Received: February 4, 2019
- Accepted: July 10, 2019
- Accepted Manuscript published: July 11, 2019 (version 1)
- Version of Record published: July 25, 2019 (version 2)
Copyright
© 2019, Santana Sanchez et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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