The mechanism of error induction by the antibiotic viomycin provides insight into the fidelity mechanism of translation
- Uppsala University, Sweden
Figures
Figure 1
Kinetics of tRNA selection in the presence of viomycin.
(A) Time courses of [3H] GDP formation by Phe-tRNAPhe containing EF-Tu TCs reacting with 0.5 µM 70S ribosomes with fMet-tRNAfMet in the P site, displaying either a cognate UUC or near-cognate CUC …
Figure 2
Stabilization of ternary complex on the ribosome by viomycin on both cognate and near-cognate codons.
(A) Time courses of chase experiments were f[3H]Met-Phe dipeptide is formed after chasing of 5 µM containing EF-TuH84A TCs from 70S ribosomes with f[3H] Met-tRNAfMet in the P site, displaying a …
Figure 3
Kinetic model for viomycin action during tRNA selection.
TC binds to a ribosome with an empty A site with rate constant k1 to form a viomycin-insensitive initial binding complex where the codon·anticodon interaction is not yet established. From this state …
Figure 4
Correlation between initial selection accuracy and viomycin sensitivity for four codon∙anticodon pairs.
(A) Time courses of f[3H]Met-Phe dipeptide formation for 1 µM Phe-tRNAPheGAA containing TCs reacting with 70S ribosomes, displaying either a cognate UUC codon or one of the near-cognate codons CUC, A…
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.46124.006
