C3H/HeN Resolved and Sensitized mice, and Adult Naive mice as a control, were infected with 108 cfu UTI89 or PBS and sacrificed at 6 or 24 hpi. (A) Bladder bacterial burdens (cfu/bladder) and (B) bladder edema were assessed in N = 4 independent experiments. Bladder edema was assessed by measuring the wet tissue weight of bladders immediately after sacrifice. (C) Levels of the cytokines CXCL1, IL-6, and CCL2 were assessed by ELISA of bladder homogenate supernatants from samples shown in panel C, which were collected from N = 3 independent experiments and assayed simultaneously in duplicate. Data points represent values for each individual mouse (the ELISA values are the average of two technical replicates), bars indicate median values, negative results are plotted at the limit of detection (dotted line). Actual P values are indicated on the graphs if >0.05, or are represented by the following symbols: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, Kruskal-Wallis test with Dunn’s correction for multiple comparisons.