Bridging the gap between single-cell migration and collective dynamics

  1. Florian Thüroff
  2. Andriy Goychuk
  3. Matthias Reiter
  4. Erwin Frey  Is a corresponding author
  1. Ludwig-Maximilians-Universität München, Germany

Abstract

Motivated by the wealth of experimental data recently available, we present a cellular-automaton-based modeling framework focussing on high-level cell functions and their concerted effect on cellular migration patterns. Specifically, we formulate a coarse-grained description of cell polarity through self-regulated actin organization and its response to mechanical cues. Furthermore, we address the impact of cell adhesion on collective migration in cell cohorts. The model faithfully reproduces typical cell shapes and movements down to the level of single cells, yet allows for the efficient simulation of confluent tissues. In confined circular geometries, we find that specific properties of individual cells (polarizability; contractility) influence the emerging collective motion of small cell cohorts. Finally, we study the properties of expanding cellular monolayers (front morphology; stress and velocity distributions) at the level of extended tissues.

Data availability

We have uploaded the source code used in the main part of our study as well as the one used in the appendix. Furthermore, we have provided the full list of parameters in the figure captions, as well as exemplary parameter files for all applicable figures.

Article and author information

Author details

  1. Florian Thüroff

    Arnold-Sommerfeld-Center for Theoretial Physics, Ludwig-Maximilians-Universität München, Munich, Germany
    Competing interests
    The authors declare that no competing interests exist.
  2. Andriy Goychuk

    Arnold-Sommerfeld-Center for Theoretial Physics, Ludwig-Maximilians-Universität München, Munich, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Matthias Reiter

    Arnold-Sommerfeld-Center for Theoretial Physics, Ludwig-Maximilians-Universität München, Munich, Germany
    Competing interests
    The authors declare that no competing interests exist.
  4. Erwin Frey

    Arnold Sommerfeld Center for Theoretical Physics, Ludwig-Maximilians-Universität München, München, Germany
    For correspondence
    frey@lmu.de
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8792-3358

Funding

German Excellence Initiative (NanoSystems Initiative Munich (NIM))

  • Erwin Frey

Deutsche Forschungsgemeinschaft (Collaborative Research Center (SFB) 1032 (project B02))

  • Erwin Frey

Deutsche Forschungsgemeinschaft (Graduate School of Quantitative Biosciences Munich (QBM))

  • Andriy Goychuk

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Pierre Sens, Institut Curie, PSL Research University, CNRS, France

Publication history

  1. Received: March 14, 2019
  2. Accepted: December 6, 2019
  3. Accepted Manuscript published: December 6, 2019 (version 1)
  4. Version of Record published: January 30, 2020 (version 2)

Copyright

© 2019, Thüroff et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Florian Thüroff
  2. Andriy Goychuk
  3. Matthias Reiter
  4. Erwin Frey
(2019)
Bridging the gap between single-cell migration and collective dynamics
eLife 8:e46842.
https://doi.org/10.7554/eLife.46842

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