Modular organization of cerebellar climbing fiber inputs during goal-directed behavior
- The University of Tokyo, Japan
- Japan Science and Technology Agency, Japan
- University of Yamanashi, Japan
- Tamagawa University, Japan
- Tokyo Medical and Dental University, Japan
- Niigata University, Japan
Figures
Figure 1 with 2 supplements
A lick/no-lick auditory discrimination task.
(A) Left, schematic diagram of a mouse performing the task under a two-photon microscope. Right, task structure. (B) Performance of an expert mouse in a representative session. Black dots represent …
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Figure 1—source data 1
Datasets used to create Figure 1.
- https://doi.org/10.7554/eLife.47021.007
Figure 1—figure supplement 1
Analyses of task performance and licking behavior.
(A) Learning curves for hit rate in all mice (n = 17). Gray dots and lines represent individual animals and magenta dots and line represent means. (B) Same as panel (A) but for CR rate. Gray dots …
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Figure 1—figure supplement 1—source data 1
Datasets used to create Figure 1—figure supplement 1.
- https://doi.org/10.7554/eLife.47021.004
Figure 1—figure supplement 2
Effects of muscimol on mouse behavior.
(A) Example behavior during a session with muscimol injection. Black dots represent licks. A vertical dashed line represents cue onset, and a gray line indicates the end of the response window. …
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Figure 1—figure supplement 2—source data 1
Datasets used to create Figure 1—figure supplement 2.
- https://doi.org/10.7554/eLife.47021.006
Figure 2 with 4 supplements
Differences in task-related CF signals between AldC+ and AldC− compartments.
(A) Schematic diagram of virus injection into the left cerebellar folium Crus II of Aldoc-tdTomato mice. Inset, magnified view of the Crus II. Red stripes represent AldC+ compartments. (B) Left, …
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Figure 2—source data 1
Datasets used to create Figure 2.
- https://doi.org/10.7554/eLife.47021.015
Figure 2—figure supplement 1
Broad correspondence between functional boundaries and AldC expression boundaries at the microzone level.
Probability of response during the early response window (0 to 0.5 s from cue onset) in single ROIs (gray dots) for each trial type (hit, magenta; FA, green; CR, blue) plotted as a function of …
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Figure 2—figure supplement 1—source data 1
Datasets used to create Figure 2—figure supplement 1.
- https://doi.org/10.7554/eLife.47021.010
Figure 2—figure supplement 2
Broad correspondence between functional boundaries and AldC expression boundaries at cellular resolution.
(A) Principal component analyses of whole ∆F/F traces from all ROIs in single imaging sessions at all AldC expression boundaries, irrespective of AldC expression. Colored dots correspond to results …
Figure 2—figure supplement 3
Task-related CF signals in individual clusters.
(A) Projection of clustering results of Figure 2—figure supplement 2A overlaid on tdTomato images at 6+/5− (top) and 5a+/4b− (bottom) boundaries. Coincidence rates of clustering results and AldC …
Figure 2—figure supplement 4
Summary of correspondence between functional boundaries and AldC expression.
(A) Number of clusters per field of view for all sessions from all mice (n = 5, 3, 8, 9, 8, 3, and two mice, 12, 4, 13, 15, 16, 13, and 6 sessions for 7+/6−, 6−/6+, 6+/5−, 5−/5+, 5+/5a−, 5a−/5a+, …
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Figure 2—figure supplement 4—source data 1
Datasets used to create Figure 2—figure supplement 4.
- https://doi.org/10.7554/eLife.47021.014
Figure 3 with 2 supplements
Difference in CF signals between the lateral and medial Crus II.
(A) Extracted ROIs representing PC dendrites that are pseudocolored overlaid on the tdTomato image around a 7+/6− (top) and a 5a+/4b− (bottom) boundary. Scale bars, 40 μm. (B) Single ROI …
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Figure 3—source data 1
Datasets used to create Figure 3.
- https://doi.org/10.7554/eLife.47021.020
Figure 3—figure supplement 1
Mediolateral separation of task-related CF signals in the Crus II.
(A) Extracted ROIs representing PC dendrites around the example 7+/6−, 6−/6+, 6+/5−, 5−/5+, 5+/5a−, 5a−/5a+, and 5a+/4b− boundaries that are pseudocolored and overlaid on the tdTomato image. Scale …
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Figure 3—figure supplement 1—source data 1
Datasets used to create Figure 3—figure supplement 1.
- https://doi.org/10.7554/eLife.47021.018
Figure 3—figure supplement 2
Difference in secondary CF signals between the lateral and medial Crus II.
(A) Percentage of responsive ROIs during the secondary response window (0.5 to 1 s from cue onset) in a compartment per session for each trial type pooled across all the lateral AldC+ or medial …
Figure 4 with 3 supplements
Behavioral relevance of pre-cue decrease in CF signals.
(A) Single trial calcium traces in a 5+ compartment with (pre-cue (↓); black) or without (no; gray) decreases in CF signals, showing difference in the pre-cue response window (–1 to 0 s from the cue …
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Figure 4—source data 1
Datasets used to create Figure 4.
- https://doi.org/10.7554/eLife.47021.026
Figure 4—figure supplement 1
Task-related CF signals in all the compartments.
(A) ROI-averaged ΔF/F traces from single compartments in single trials were classified by trial types and averaged across sessions and animals. Thick colored lines represent mean and the shadings …
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Figure 4—figure supplement 1—source data 1
Datasets used to create Figure 4—figure supplements 1 and 3.
- https://doi.org/10.7554/eLife.47021.023
Figure 4—figure supplement 2
Single ROI single trial CF signals.
(A) Single trial calcium traces for single ROI in an example imaging session from a 5+ compartment. Trials detected as pre-cue decreases (P(↓)), early increases (E), and secondary increases (S) in …
Figure 4—figure supplement 3
CF signals at late timing during licking trials and nontask licking epochs.
(A) ROI-averaged ΔF/F traces from single compartments in single licking trials (hit and FA) and nontask licking epochs were classified by trial types and averaged across sessions and animals. Thick …
Figure 5
Behavioral relevance of early increase in CF signals.
(A) Single trial calcium traces in a 5+ compartment with (early (+); black) or without (no; gray) increases in CF signals showing a difference in the early response window (0 to 0.5 s from cue …
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Figure 5—source data 1
Datasets used to create Figure 5.
- https://doi.org/10.7554/eLife.47021.028
Figure 6
| Behavioral relevance of secondary increase in CF signals.
(A) Single trial calcium traces in a 5a− compartment with (2nd (+); black) or without (no; gray) increases in CF signals showing difference during the secondary response window (0.5 to 1 s from cue …
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Figure 6—source data 1
Datasets used to create Figure 6.
- https://doi.org/10.7554/eLife.47021.030
Figure 7
Late changes in CF signals represent positive reward outcomes.
(A) Single trial calcium traces in a 6− compartment with (late (↓); black) or without (no; gray) decreases in CF signals during the late response window aligned to lick onset during licking trials …
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Figure 7—source data 1
Datasets used to create Figure 7.
- https://doi.org/10.7554/eLife.47021.032
Figure 8
Diversity of CF signals across the entire Crus II and their functional implications.
A schematic of our main findings. Red and white stripes represent AldC+ and AldC− compartments, respectively. A dashed line represents the functional boundary of lateral and medial Crus II. Red and …
Tables
Key resources table
| Reagent type | Designation | Source | Identifier |
|---|---|---|---|
| Recombinant DNA reagent | pENN.AAV1.CMVs.PI.Cre.rBG | Addgene | RRID: Addgene_105537 |
| Recombinant DNA reagent | pAAV1.CAG.Flex.GCaMP6f.WPRE.SV40 | Addgene | RRID: Addgene_100835 |
| Genetic Reagent (Mus musculus) | C57BL/6NCrSlc | Japan SLC, Inc | RRID: MGI:5295404 |
| Genetic Reagent (Mus musculus) | Aldoc-tdTomato KI, C57BL/6N-Aldoc<tm1(tdTomato)Ksak> | RIKEN BioResource Center | MGI:6324252 |
| Software, algorithm | Matlab | MathWorks | RRID: SCR_001622 |
| Software, algorithm | Labview | National Instruments | RRID: SCR_014325 |
| Software, algorithm | Suite2P | GitHub | https://github.com/cortex-lab/Suite2P |
| Software, algorithm | Lick/no-lick task | GitHub | https://github.com/stsutsumi223/Aldoc-behavior |
Additional files
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Supplementary file 1
Statistics results.
Complete statistics results for all the figures.
- https://doi.org/10.7554/eLife.47021.034
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Transparent reporting form
- https://doi.org/10.7554/eLife.47021.035
