Modular organization of cerebellar climbing fiber inputs during goal-directed behavior
- The University of Tokyo, Japan
- Tamagawa University, Japan
- Niigata University, Japan
- University of Yamanashi, Japan
Abstract
The cerebellum has a parasagittal modular architecture characterized by precisely organized climbing fiber (CF) projections congruent with alternating aldolase C/zebrin II expression. However, behavioral relevance of CF inputs to individual modules remains poorly understood. Here, we used two-photon calcium imaging in the cerebellar hemisphere Crus II in mice performing an auditory go/no-go task to investigate the functional differences in CF inputs to modules. CF signals in medial modules show anticipatory decreases, early increases, secondary increases, and reward-related increases or decreases, which represent quick motor initiation, go cues, fast motor behavior, and positive reward outcomes. CF signals in lateral modules show early increases and reward-related decreases, which represent no-go and/or go cues and positive reward outcomes. The boundaries of CF functions broadly correspond to those of aldolase C patterning. These results indicate that spatially segregated CF inputs in different modules play distinct roles for execution of goal-directed behavior.
Data availability
Data analysed for all the figures are included in the manuscript and source data files. The Aldoc-tdTomato mouse line will be available at RIKEN Bio Resource Center.
Article and author information
Author details
Masanori Matsuzaki
Funding
Japan Society for the Promotion of Science (23115504)
- Kazuo Kitamura
Japan Society for the Promotion of Science (25115705)
- Kazuo Kitamura
Japan Society for the Promotion of Science (25290003)
- Kazuo Kitamura
Japan Society for the Promotion of Science (15H01426)
- Kazuo Kitamura
Japan Society for the Promotion of Science (17H03543)
- Kazuo Kitamura
Japan Society for the Promotion of Science (17H06313)
- Kazuo Kitamura
Japan Society for the Promotion of Science (21220006)
- Masanobu Kano
Japan Society for the Promotion of Science (25000015)
- Masanobu Kano
Japan Society for the Promotion of Science (18H04012)
- Masanobu Kano
Ministry of Education, Culture, Sports, Science, and Technology (Comprehensive Brain Science Network)
- Kenji Sakimura
- Masanobu Kano
- Kazuo Kitamura
Ministry of Education, Culture, Sports, Science, and Technology (Brain Information Dynamics)
- Kazuo Kitamura
Ministry of Education, Culture, Sports, Science, and Technology (The Strategic Research Programme for Brain Sciences)
- Masanobu Kano
Japan Agency for Medical Research and Development (Brain/MINDS)
- Masanobu Kano
- Kazuo Kitamura
Takeda Science Foundation
- Kazuo Kitamura
Uehara Memorial Foundation
- Kazuo Kitamura
Japan Society for the Promotion of Science (25560432)
- Kazuo Kitamura
Japan Society for the Promotion of Science (25113705)
- Kazuo Kitamura
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All experiments were approved by the Animal Experiment Committees of the University of Tokyo (#P08-015) and University of Yamanashi (#A27-1).
Copyright
© 2019, Tsutsumi et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Modular organization of cerebellar climbing fiber inputs during goal-directed behavior
eLife 8:e47021.
https://doi.org/10.7554/eLife.47021
