For most adult mammals, the risk of death increases exponentially with age, an observation originally described for humans by Benjamin Gompertz. We recently performed a Kaplan–Meier survival analysis of naked mole-rats (Heterocephalus glaber) and concluded that their risk of death remains constant as they grow older (Ruby et al., 2018). Dammann et al. suggest incomplete historical records potentially confounded our demographic analysis (Dammann et al., 2019). In response, we applied the left-censorship technique of Kaplan and Meier to exclude all data from the historical era in which they speculate the records to be confounded. Our new analysis produced indistinguishable results from what we had previously published, and thus strongly reinforced our original conclusions.https://doi.org/10.7554/eLife.47047.001
Dammann et al. express concern that only a small fraction of the naked mole-rat lifespan (4400 days, or ~12 years) has been used to conclude that hazard rates are constant (Dammann et al., 2019). This concern derives from the preternaturally long lifespans experienced by an apparently non-aging mammal in a relatively safe, captive environment: at ages far beyond their allometrically-expected lifespans, or their lifespans in the wild, only a minority of the naked mole-rat population has expired. Unlike for organisms with Gompertzian hazard, the concept of a maximal lifespan is impossible to define meaningfully for a non-aging species, a conundrum that was discussed as follows in our original paper: “Under an exponential decay model, the concept of a ‘maximal lifespan’ loses relevance: at no point does mortality hazard grow into an insurmountable obstacle. One could try and force the concept of ‘maximal lifespan’ onto an exponentially-decaying species by defining it in terms of a high percentile of lifespan (say, 95%) – and by that definition, the maximal lifespan of the naked mole-rat would be very long indeed! But that definition ignores the implicit meaning of the term: as an age beyond which biological wear and damage has become insurmountable” (Ruby et al., 2018).
The question remains: how long must a lack of aging be observed in order to be considered long-enough? Dammann et al. remain attached to the notion of maximal lifespan, despite the logical conundrum discussed above. In contrast, we considered the span of time across which our conclusions were irrefutable (4400 days, or ~12 years) in the contexts of their allometrically-expected full lifespans, or their lifespans as measured in the wild: “That twelve year stretch of time can be considered from several perspectives. In Figure 5, it is considered from the perspective of Tsex, that is the minimum lifespan allowable for an organism to reproduce. Most mammals live beyond that age, as is required to provide progeny with support and nurturing (Lee, 2003), but show signs of demographic aging within a few fold of Tsex; from this perspective, the naked mole-rat is exceptional, showing no signs of demographic aging many dozens-of-fold beyond Tsex (Figure 5). Twelve years can also be considered in the context of the expected total lifespan of a naked mole-rat based on its body size (de Magalhães et al., 2007): this 35-gram rodent would be expected to live up to six years, but instead has not shown the first sign of demographic aging at twice that age. Twelve years could finally be considered from the perspective of natural lifespan in the wild, which is estimated to be 2–3 years for the naked mole-rat (Hochberg et al., 2016)” (Ruby et al., 2018).
The argument in Ruby et al. (2018) relies on observations within the first 12 years of life, which means that the objections of Dammann et al. to the sizes of error bars at older ages are not relevant (though we stand behind our decision to communicate the full scope of available data). But even if one is not satisfied by our arguments, we seriously considered these concerns in our original manuscript, discussing them at length: “We cannot reject the possibility of an as-yet-undetectable Gompertzian component to naked mole-rat mortality. And in fact, this alternative model is not formally falsifiable: any observational extension of constant hazard would simply delay the earliest point at which Gompertzian hazard might take effect. However, given the extremely low baseline hazard rate of 1/10,000 per day for non-breeders and ~1/100,000 for breeders, it seems unlikely that the Gompertzian hazard is being overwhelmed. But even under a delayed-aging model, naked mole-rats profoundly distinguish themselves from other mammals: in this context, through an unprecedentedly-long adulthood prior to the emergence of Gompertzian hazard” (Ruby et al., 2018).
Dammann et al. express the concern that “the data set could be biased due to missing death records before 2008” (Dammann et al., 2019). To address this concern, we have re-analyzed our published data including only observations made on or after January 1, 2008. For animals born prior to that date, observational data (of them being alive) was truncated using left-censorship (that is, truncation on the left). This approach is recommended in the original publication paper by Kaplan and Meier, where it is deemed appropriate “if it is desired to permit the entrance of items [i.e. animals] into the sample after the commencement of their lifetimes” (page 463 of Kaplan and Meier, 1958). Such is the case here. Dammann et al. assert that “methods for addressing left censoring require information on the number of missing animals”. To the contrary, Kaplan and Meier explicitly describe the lack of such information as an assumption of their technique: “It is assumed that nothing is known of the existence of any such item [i.e. animal] that dies before it becomes available for observation” (Kaplan and Meier, 1958). Since Dammann et al. do not question the validity of data collected in or after 2008, left truncation at January 2008 should address their concern of alleged bias.
We applied this standard technique, and Figure 1A shows juxtaposed Kaplan–Meier survival analysis with left-censorship at January 1, 2008 (orange) along with our original Kaplan–Meier analysis (Figure 1 of Ruby et al., 2018; green). The survival plot was minimally affected by this new analytical framework, with final survival still above 50% (ending at 51.9% rather than 61.6%). The new analysis continued to match the expected trajectory for constant daily mortality hazard of 8 × 10−5 (Figure 1A; dashed purple line). The differences between our original and new analyses were therefore insufficient to warrant any modification to our original conclusions about the median lifespan for Tsex-surviving naked mole-rats being ~6900 days (19 years).
We used the left-censored Kaplan–Meier survival curve to calculate the age-specific mortality hazard across nine age bins (Figure 1B, orange). Those new estimates failed to show an increase with age and were statistically indistinct versus the age-specific hazards presented in our original manuscript (Figure 1B, green). We therefore maintain our original conclusion: that naked mole-rat mortality hazard does not increase with age, with that assertion being statistically robust to the beginning of our final estimate bin (6,529 days, or 18 years).
The central finding of Ruby et al. (2018) – that naked mole-rats defy Gompertzian mortality laws – was based on Kaplan–Meier survival and hazard analyses on all the high-quality, full-lifespan data at our disposal. We followed that up with analyses inclusive of low-quality (imprecise) data (Figure 2 of Ruby et al., 2018), as well as independent analyses of each sex and identifiable social class (i.e. breeders, non-breeders) of naked mole-rats (Figures 3 and 4 of Ruby et al., 2018). Each of those analyses reinforced our original conclusion. Further, to address the concern of Dammann et al. that our records from pre-2008 could potentially have introduced bias to our analyses, we performed a new analysis. In light of the robust agreement between our original analysis (Ruby et al., 2018) and our new analysis, we stand by all of the conclusions in Ruby et al. (2018).
Kaplan–Meier survival analysis was performed on the data from Supplementary file 1 from Ruby et al. (2018), filtered as described for Figure 1 of that paper (the ‘initial analysis’: animals with day-resolution birth and death/censorship data), again according to the method of Kaplan and Meier (1958), but now truncating lifespan observations on the left at January 1, 2008. For example: an animal A born on after January 1, 2008 was considered identically as in Ruby et al. (2018). In contrast, an animal B born one year prior (on January 1, 2007), would not have been counted as a member of the population until day 365. For that animal, as expressed by equations 2a and 2b of Kaplan and Meier (1958), one count representing that animal would be added to nj at j = 365.
Age-specific per-day hazard was calculated across bins (as described in Ruby et al., 2018), with left-censorship again taken into account. Using the examples above: animal A would be considered identically to the original analysis. For the first bin (days 183 through 399), if animal A survived that bin, then it would have contributed zero deaths to the numerator and 217 days to the denominator of that bin (i.e. the length of the bin); had it died at 380 days, then it would have contributed one death to the numerator and 198 days to the denominator (the number of days it was observed to be alive in that bin, plus the day it died). In contrast, if animal B had survived that bin, it would have contributed only 35 days to the denominator (its entry point at day 365 through day 399). Had animal B died at 380 days, then it would have contributed one death to the numerator and only 16 days to the denominator (the number of days it was observed alive post-truncation, plus the day it died).
All data used in this paper were published as part of Ruby et al., 2018.
An analysis of the relationship between metabolism, developmental schedules, and longevity using phylogenetic independent contrastsThe Journals of Gerontology Series A: Biological Sciences and Medical Sciences 62:149–160.https://doi.org/10.1093/gerona/62.2.149
Nonparametric estimation from incomplete observationsJournal of the American Statistical Association 53:457–481.https://doi.org/10.1080/01621459.1958.10501452
Michael RoseReviewing Editor; University of California, Irvine, United States
Patricia J WittkoppSenior Editor; University of Michigan, United States
Laurence MuellerReviewer; UC Irvine, United States
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
Thank you for submitting your article 'Response to comment on 'Naked mole-rat mortality rates defy Gompertzian laws by not increasing with age' to eLife. Your article has been reviewed by two peer reviewers, and the evaluation has been overseen by a Reviewing Editor (Michael Rose), a Senior Editor (Patricia Wittkopp), and the eLife Features Editor (Peter Rodgers). The following individuals involved in review of your submission have agreed to reveal their identity: Laurence Mueller (Reviewer #1); Caleb Finch (Reviewer #2).
The reviewers supported publication of your article subject to the following point being addressed in a revised version.
1) Dammann et al. raise the concern that only a small fraction of the lifespan has been used to conclude hazard rates are constant. Even with this small range, the confidence interval on hazard rates in the last age range are quite large. The authors of this Response should address this concern.https://doi.org/10.7554/eLife.47047.005
1) Dammann et al. raise the concern that only a small fraction of the lifespan has been used to conclude hazard rates are constant. Even with this small range, the confidence interval on hazard rates in the last age range are quite large. The authors of this Response should address this concern.
We once again address this discussion point in our revised manuscript and highlight where in our original paper we had originally discussed the concerns that have been re-raised by Dammann et al. in their comment. We have added substantial text addressing this concern, largely by restating points made in the Discussion section of the original paper (Ruby et al., 2018). As before, our re-analyses of the data using left censorship (submitted in the 1st draft of the commentary) strongly support our original conclusions.https://doi.org/10.7554/eLife.47047.006
- Rochelle Buffenstein
The authors were all employees of Calico Life Sciences, LLC at the time the study was conducted.
- Patricia J Wittkopp, University of Michigan, United States
- Michael Rose, University of California, Irvine, United States
- Laurence Mueller, UC Irvine, United States
- Received: March 21, 2019
- Accepted: June 26, 2019
- Version of Record published: July 9, 2019 (version 1)
© 2019, Ruby et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
The longest-lived rodent, the naked mole-rat (Heterocephalus glaber), has a reported maximum lifespan of >30 years and exhibits delayed and/or attenuated age-associated physiological declines. We questioned whether these mouse-sized, eusocial rodents conform to Gompertzian mortality laws by experiencing an exponentially increasing risk of death as they get older. We compiled and analyzed a large compendium of historical naked mole-rat lifespan data with >3000 data points. Kaplan-Meier analyses revealed a substantial portion of the population to have survived at 30 years of age. Moreover, unlike all other mammals studied to date, and regardless of sex or breeding-status, the age-specific hazard of mortality did not increase with age, even at ages 25-fold past their time to reproductive maturity. This absence of hazard increase with age, in defiance of Gompertz’s law, uniquely identifies the naked mole-rat as a non-aging mammal, confirming its status as an exceptional model for biogerontology.
Groups of animals inhabit vastly different sensory worlds, or umwelten, which shape fundamental aspects of their behaviour. Yet the sensory ecology of species is rarely incorporated into the emerging field of collective behaviour, which studies the movements, population-level behaviours, and emergent properties of animal groups. Here, we review the contributions of sensory ecology and collective behaviour to understanding how animals move and interact within the context of their social and physical environments. Our goal is to advance and bridge these two areas of inquiry and highlight the potential for their creative integration. To achieve this goal, we organise our review around the following themes: (1) identifying the promise of integrating collective behaviour and sensory ecology; (2) defining and exploring the concept of a ‘sensory collective’; (3) considering the potential for sensory collectives to shape the evolution of sensory systems; (4) exploring examples from diverse taxa to illustrate neural circuits involved in sensing and collective behaviour; and (5) suggesting the need for creative conceptual and methodological advances to quantify ‘sensescapes’. In the final section, (6) applications to biological conservation, we argue that these topics are timely, given the ongoing anthropogenic changes to sensory stimuli (e.g. via light, sound, and chemical pollution) which are anticipated to impact animal collectives and group-level behaviour and, in turn, ecosystem composition and function. Our synthesis seeks to provide a forward-looking perspective on how sensory ecologists and collective behaviourists can both learn from and inspire one another to advance our understanding of animal behaviour, ecology, adaptation, and evolution.