(A-C) Animals were fed dsRNA for two weeks, amputated to remove heads and tails, and allowed to regenerate for 15 days. Regenerating head fragments were fixed and stained for gad expression, regenerating tail fragments were stained for ppl-1, trpA, opsin/tyrosinase, glut, or if-1/cali expression, and regenerating trunks were stained for chat or dd17258 expression. Cell types amenable to total animal enumeration were quantified by normalizing cell number to body size by dividing by the square root of whole animal area (gad+, brain and pharynx ppl1+, brain sert+, dd17258+, opsin+ cells). Abundances of cell types too numerous for whole-body counting were quantified by manually scoring cell numbers in a region of interest and normalizing to the area of that region (trpA+ cells were scored in an anterolateral region, peripheral chat+ neurons scored in a postpharyngeal ventromedial region, if-1+;cali, and glut+ cells scored within the brain defined by Hoechst staining). (A) tec-1 RNAi animals had increased numbers of gad+ neurons, ppl-1+ neurons within the brain, dd17258+ neurons, sert+ brain neurons, trpA+ peripheral neurons, and chat+ peripheral neurons. (B) tec-1(RNAi) animals regenerated disorganized photoreceptors but had no alteration in opsin+ cell numbers. Likewise, tec-1 inhibition did not alter numbers of pharyngeal ppl-1+ neurons. (C) tec-1 knockdown decreased the density of glut+ and pooled if-1/cali+ glial cells in the brain. Significance determined by two-tailed t-tests (*, p<0.05; ***, p<0.001; n.s. p>0.05). Images show Hoechst-stained maximum projections except for maximum-projected pharyngeal ppl-1+ cells shown for clarity overlayed with a single slice of Hoechst-labeled pharynx tissue. Scale bars: 100 μm unless otherwise noted.