(A) Most licks in FamAve are within a 1 s window centered on reward delivery (defined as ‘correct’ licks). The fraction of ‘correct’ licks is initially lower in New relative to Fam but increases over days. (B) The fraction of incorrect, unrewarded, entries into a track endzone which coincide with a bout of licking across five days of remapping. Mice are significantly more likely to lick in expectation of a reward upon incorrectly entering an endzone in the familiar environment (when they return to the same reward zone twice in a row), suggesting that they know the reward locations in Fam but not New. This difference decreases over the course of exposure to the New world. (C) Mice lick at lower rates in New. (D) Mean distance from endzone of missed trial, identified as the location of a premature turn in the track. Failed trials (when animals turn around too early in the track and return to the same end zone, resulting in no reward) in the New world occur further from the correct end zones than they do in the familiar environment. This difference decreases over 5 days of remapping. (E) Mice spend a similar amount of time stopped in both environments over 5 days, indicating that stopped periods do not significantly contribute to decreased behavioral performance. (F) Running speed in New across five days of remapping, normalized to mean speed in FamAve. Average running speed is the same in FamAve and New, suggesting slower running does not contribute to decreased behavioral performance. (G) Recovery of behavioral performance does not accelerate when animals undergo a second sequence of remapping into another distinct new world (New 2). Performance in New 1 (original new environment) normalized to flanking FamAve was compared to performance in New 2 (second distinct new environment) across 5 days (N = 6, a subset of animals used in other panels, p>0.99 by paired sample t-test with Bonferroni-Holm Corrections). (H) Mice were initially impaired in behavioral performance in the new environment relative to both Fam and Fam′, but the data show return to high performance in Fam′, suggesting that satiation or fatigue do not contribute to impaired behavioral performance in New. Mean performance was measured in terms of rewards per minute in the New world over 5 days of remapping. Black asterisks represent Fam vs New, Gray asterisks represent Fam′ vs New. All Fam vs Fam′ comparisons are not significantly different. (N = 14 for all panels except (G); *p<0.05, **p<0.01, ***p<0.001 by paired sample t-test or one-sample t-test with Bonferroni-Holm corrections).