Cancer and normal samples of (A) M. chilensis from Argentina and (B) M. edulis from Europe were analyzed for the presence of 13 SNPs that are diagnostic for M. trossulus alleles. The fraction of the fluorescence attributable to the M. trossulus allele is shown for each SNP, and the mean multi-locus estimate is shown on the right. SNP IDs indicate shortened names of the specific nuclear SNP loci used (see Supplementary file 3). Red points indicate M. chilensis and blue indicate M. edulis. Dark filled circles represent cancer samples, dark open circles represent normal samples which were a part of the sequence and qPCR analysis (Figures 1–4), and light open circles represent samples from an extended set of normal individuals from the same populations. Cancer samples included: Mch23, Mch41, and Mch42 for M. chilensis, and BA30, HO8, Ch5, Ch13, LA84, and STBRI43 from M. edulis. Normal samples included Mch3, Mch5, and Mch7 for M. chilensis, and HO9, HO19, Ch10, and Ch11 for M. edulis, as well as 109 additional normal M. chilensis and 62 additional normal M. edulis. See Figure 6—source data 1 for M. chilensis data and Figure 6—source data 2 for M. edulis data.