Cell line (Mus musculus) | Primary Bone Marrow Derived Macrophage Cells from Phlpp1+/+ mice | This paper | WT BMDM | male, 6–8 week old C57BL/6 mice, Charles River Laboratories |
Cell line (Mus musculus) | Primary Bone Marrow Derived Macrophage Cells from Phlpp1-/- mice | This paper | Phlpp1-/- BMDM | male, 6–8 week old C57BL/6 mice, Charles River Laboratories |
Cell line (Mus musculus) | Immortalized MEFs from Phlpp1+/+ mice | Lloyd Trotman Lab, CSHL, PMID: 21840483 | | stably expressing shp53 and GFP |
Cell line (Mus musculus) | Immortalized MEFs from Phlpp1-/- mice | Lloyd Trotman Lab, CSHL, PMID: 21840483 | | stably expressing shp53 and GFP |
Cell line (Homo sapiens) | HeLa | ATCC | CCL-2 | |
Cell line (Homo sapiens) | HEK-293T | ATCC | CRL-11268 | |
Cell line (Mus musculus) | NCTC clone L929 | ATCC | CCL-1 | L cell, L-929, derivative of Strain L |
Strain, strain background (Escherichia coli) | E. coli K1 strain RS218 | PMID: 6995336 | | Victor Nizet Lab, University of California San Diego |
Strain, strain background (Mus musculus) | WT and littermate control Phlpp1-/- mice | PMID: 20080691 | | female, 10 to 14 week old, C57BL/6, for mouse infection studies |
Antibody | anti-HA (rat, monoclonal) | Roche | 11867425001 | Western Blot (1:1000) |
Antibody | anti-GFP (rabbit, polyclonal) | Cell Signaling | 2555 | Western Blot (1:1000) |
Antibody | anti-STAT1 (rabbit, polyclonal) | Cell Signaling | 9172 | Western Blot (1:1000) |
Antibody | anti-STAT1 pSer727 (rabbit, polyclonal) | Cell Signaling | 9177 | Western Blot (1:1000) |
Antibody | anti-STAT1 pTyr701 (rabbit, polyclonal) | Cell Signaling | 7649 | Western Blot (1:1000) |
Antibody | anti-Erk1/2 pThr202/pTyr204 (rabbit, polyclonal) | Cell Signaling | 9101 | Western Blot (1:1000) |
Antibody | anti-Erk1/2 (rabbit, polyclonal) | Cell Signaling | 9102 | Western Blot (1:1000) |
Antibody | anti-Akt pThr308 (rabbit, polyclonal) | Cell Signaling | 9275 | Western Blot (1:1000) |
Antibody | anti-Akt (rabbit, polyclonal) | AbCam | 126811 | Western Blot (1:1000) |
Antibody | anti-PHLPP1 (rabbit, polyclonal) | Proteintech | 22789–1-AP | Western Blot (1:1000) |
Antibody | anti-FLAG (mouse, monoclonal) | Sigma-Aldrich | F3165 | Western Blot (1:1000) |
Antibody | anti-β-Actin (mouse, monoclonal) | Sigma-Aldrich | A2228 | Western Blot (1:2000) |
Antibody | anti-α-tubulin (mouse, monoclonal) | Sigma-Aldrich | T6074 | Western Blot (1:1000) |
Antibody | anti-STAT1 (rabbit, polyclonal) | Santa Cruz Biotechnology | sc-345 | Chromatin Immunoprecipitation |
Antibody | anti-HA (mouse, monoclonal) | BioLegend | 901503 | Immunoprecipitation |
Antibody | anti-α-tubulin (rabbit, monoclonal) | Cell Signaling | 2125 | Immunofluorescence (1:200) |
Antibody | anti-Alexa647 (goat anti-Mouse IgG) | Life Technologies | A21235 | Immunofluorescence (1:500) |
Antibody | anti-Alexa477 (goat anti-Rabbit IgG) | Life Technologies | A11034 | Immunofluorescence (1:500) |
Recombinant DNA reagent | pCMV 3XFLAG-PHLPP1 WT | This paper | | residues 1–1717 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-NLS-PHLPP1 PP2C | This paper | | residues 653–906 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-NLS-PHLPP1 PP2C D1210/1413A | This paper | DDAA | Aspartate at residues 1210 and 1413 mutated to Alanine |
Recombinant DNA reagent | pCDNA3 HA PHLPP1 NTE-WT | This paper | | residues 1–512 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-PHLPP1 NTE-NLS1 mut. | This paper | | residues 1–512 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-PHLPP1 NTE-NLS2 mut. | This paper | | residues 1–512 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-PHLPP1 NTE-NLS1/2 mut. | This paper | | residues 1–512 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-NES-PHLPP1 NTE | This paper | | residues 1–512 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-PHLPP1 ΔNTE | PMID: 15808505 | Addgene: 22404 | residues 513–1717 of PHLPP1 |
Recombinant DNA reagent | pCDNA3 HA-PHLPP1NES-PHLPP1 NTE | This paper | | residues 1–512 of PHLPP1 |
Sequence-based reagent | Stat1 SMART siRNA pools | Dharmacon | L-058881 | |
Sequence-based reagent | Control SMART siRNA pools | Dharmacon | D-001810-10-05 | |
Peptide, recombinant protein | murine IFNγ | PeproTech | 315–05 | 10 ng/mL |
Peptide, recombinant protein | human IFNγ | PeproTech | 300–02 | 10 ng/mL |
Peptide, recombinant protein | recombinant STAT1 | Biosource | PHF0011 | 0.3 uM |
Peptide, recombinant protein | recombinant human Cdk1/CyclinB | Millipore | 14–450 | 0.2 uM |
Peptide, recombinant protein | E. coli O111:B4 LPS | Sigma-Aldrich | L4391 | |
Chemical compound, drug | Okadaic Acid (OA) | Millipore | 459616 | 1 µM |
Chemical compound, drug | Gö6983 | Calbiochem | 365251 | 250 nM |
Chemical compound, drug | Staurosporine | Calbiochem | 569397 | 1 µM |
Chemical compound, drug | KLA | Avanti Polar Lipids | 699500 | 100 ng/mL |
Chemical compound, drug | RO-3306 | Enzo | ALX-270–463 | 144 uM |
Commercial assay or kit | Dual-Glo Luciferase Assay System | Promega | E2940 | |
Commercial assay or kit | IL-6 ELISA Kit | R and D Systems | DY406 | |
Commercial assay or kit | IL-1β ELISA Kit | R and D Systems | DY401 | |
Commercial assay or kit | IL-10 ELISA Kit | R and D Systems | DY417 | |