(a) Hoffman modulated contrast image of acute telencephalic slice of E14.5 mouse brain showing patch clamp electrode (green outline) holding a tdTomato fluorescent Nkx2.1+ MGE-derived GABAergic interneuron in the perforated-patch voltage clamp configuration, with a multi-barrel drug pipette (red outline) positioned in the vicinity. (b) EGABA was empirically determined by focal application of GABA (black bars, 50 µM) at varying holding potentials under control (black traces), and ethanol exposure (6.5 mM EtOH, red traces), conditions. Dotted lines indicate EGABA for each condition, scale bar vertical = 10 pA; horizontal = 500 ms. (c) I/V plot of peak GABA-induced current over holding potential defines EGABA as the x-intercept under control (black), ethanol exposure (red), and aCSF wash (gray) conditions. Dotted blue line denotes mean resting membrane potential. (d) EGABA of neurons under control conditions (aCSF), during ethanol exposure (EtOH+aCSF), and aCSF wash (Wash). ** = p < 0.01 one-way ANOVA with Dunnett post-test. (e) Group-wise comparison of EGABA in Nkx2.1+ neurons without and with bumetanide pretreatment (Bumet, 20 µM) and during exposure to EtOH with bumetanide pretreatment (EtOH+Bumet). Numbers below scatter denote number of litters and number of cells recorded in (). *, ** = p < 0.05, p < 0.01 compared to aCSF, one-way ANOVA with Dunnett post-test. (f) Magnitude of change in EGABA induced by 6.5 mM ethanol without and with bumetanide pretreatment (p > 0.05, unpaired t-test).