1. Developmental Biology
  2. Stem Cells and Regenerative Medicine
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A distinct transition from cell growth to physiological homeostasis in the tendon

  1. Mor Grinstein
  2. Heather L Dingwall
  3. Luke D O'Connor
  4. Ken Zou
  5. Terence Dante Capellini
  6. Jenna Lauren Galloway  Is a corresponding author
  1. Massachusetts General Hospital, Harvard Medical School, United States
  2. Harvard University, United States
  3. Broad Institute of Harvard and MIT, United States
  4. Harvard Stem Cell Institute, United States
Research Article
Cite this article as: eLife 2019;8:e48689 doi: 10.7554/eLife.48689
6 figures, 5 videos, 1 table and 5 additional files

Figures

Figure 1 with 1 supplement
H2B-GFP expression is induced upon the addition of Dox to timed pregnant females from E10 to P0 (dark green); Dox is removed for the chase period of 0–2 years and H2B-GFP protein is diluted (light green) in proportion to cell division.

(A) At birth (P0), longitudinal sections of Achilles (B–B’’) tendons (n = 3 mice) show extensive H2B-GFP+ (green B, B'; white B’’) labeling of Hoechst+ nuclei (blue, B, B’). SHG is shown in white (B, C). Histogram showing that more than 95% of the cells are H2B-GFP+ at P0 (D). After 680 days, Achilles (C–C’’) tendons (n = 3 mice) have qualitatively fewer H2B-GFP+ (green C, C'; white C’’) labeled Hoechst+ nuclei (blue C, C’). Histogram showing only 20% of the cells are H2B-GFP+ and the H2B-GFP intensity has decreased at 645 days (E). For the histograms, a representative is shown; tendons from n > 3 mice were examined independently. Scale Bars, 50 µm; Vertical red lines (H, O) indicate the control GFP beads for standardizing intensity and gates.

https://doi.org/10.7554/eLife.48689.003
Figure 1—figure supplement 1
Flow cytometry analysis of H2B-GFP+ tendon cells showing the exclusion of CD31+ and CD45+ cells; CD31-/CD45- cells (80–90%) were used for our H2B-GFP analysis.

(A) A representative flow cytometry analysis of H2B-GFP expression in a H2B-GFP heterozygous mouse that never received Dox, indicating a relatively small amount (<1%) of transgene expression occurs in the absence of Dox induction (B). n = 3 mice per experiment.

https://doi.org/10.7554/eLife.48689.004
Analysis of H2B-GFP+ cells from postnatal to aged stages.

The percentage of H2B-GFP+ tendon cells decreases from P0 to P645, with a rapid decline of H2B-GFP+ tendon cells by 14 days post birth and a more graduate decrease in H2B-GFP+ cells from P14 to P645 (A). Analysis of H2B-GFP+ tendon cells shows logarithmic decay (log2) in the percentage of H2B-GFP+ cells (B). H2B-GFP+ tendon cells decrease in their intensity of GFP from P0 to P645 (C; stages examined are labeled and shown with different shades of green; histogram is a representative from n = 4 mice). Using the change in H2B-GFP intensity, a model was used to calculate the daily cell proliferation rates (D), which shows significantly higher proliferation rates from P0 to P21, and lower proliferation rates at P80 and P600. Tendons from n > 3 mice were examined per each stage shown on the graph. All error bars represent standard deviation.

https://doi.org/10.7554/eLife.48689.005
Figure 3 with 1 supplement
Analysis of tendon cell proliferation using short and long pulses of BrdU/EdU.

Examination of BrdU incorporation 24 hr after BrdU injection reveals higher BrdU incorporation rates at P0 through P14 compared with P21 and later stages (A). Representative flow cytometry of 24 hr BrdU labeled Scx-Cre;TdTom+ tendon cells shows 30.3% BrdU+/TdTom+ cells at P8 and 6.65% BrdU+/TdTom+ cells at P28 (B). 24 hr EdU labeling identifies a significant number of proliferating cells at P2 (C, A’, B’), but no EdU+ cells were observed at P60 (C, C’,D’). Administration of BrdU for over 90 days after P30 shows low but detectable levels of BrdU incorporation from 120 to 400 days (D). Example flow cytometry shows 2.18% BrdU+/TdTom+ cells after 3 months of BrdU incorporation (1–4 months, (E). Quantification of BrdU+/TdTom+ cells in tendon sections (F) shows a similar percentage of incorporation as the flow cytometry analysis and significantly more BrdU incorporating nuclei in outer Achilles tendon regions compared with inner regions at either 3 and 6 months. For all experiments, n = 3 mice were examined per stage. Error bars are standard deviation. Scale bars = 100 µm.

https://doi.org/10.7554/eLife.48689.008
Figure 3—figure supplement 1
No significant differences in the percentage of BrdU+ cells were observed between Scx-Cre;TdTom+ cells and Scx-GFP+ cells after 120 days of BrdU incorporation.

n = 3 mice per experiment. Error bars are standard deviation.

https://doi.org/10.7554/eLife.48689.009
Expression of tendon and matrix related genes changes during the transition in cell division rate.

RT-qPCR of selected markers of proliferation (Mki67; blue), tendon transcription factors (TFs, Scx and Mkx; green), and extracellular matrix (Col1a2, Col3a1, and Fmod; gray). Relative expression was calculated using the ∆CT method using Gapdh as the reference gene. For all genes assayed, significant differences between all six time points were found via ANOVA (p<0.05). Stars indicate significant differences based on Tukey’s HSD compared to P0 only (*p<0.05; **p<0.01; ***p<0.001). See Supplementary file 1 for ANOVA statistics and full report of post hoc pairwise comparisons. n = 3 biological replicates per time point. Boxplot edges represent the interquartile range (IQR) and the middle line represents the median. Whiskers represent 1.5 x IQR.

https://doi.org/10.7554/eLife.48689.011
Tendon growth corresponds to changes in tendon cell density and matrix organization.

Two photon microscopy images of Achilles tendons showing second harmonic generation signal (purple, A-D’; white, A’’’–D’’’), Hoechst+ nuclei (white, A–D’), and Scx-GFP+ cells (green, A–D, A’’–D’’). Images show changes in cell density, cell shape, and collagen organization at P0 (A–A’’’), P7 (B–B’’’), P14 (C–C’’’), and P28 (D–D’’’). Using optical sections from 2-photon images, we found that tendon cell density significantly decreases at postnatal stages (E, each point represents cell counts from an optical section; n = 3 mice analyzed per stage). The Achilles tendon undergoes significant longitudinal growth from P0 to P30; no significant increases in Achilles length is detected after P30 (F, each point represents measurements from one mouse Achilles; n > 3 mice). Error bars are standard deviation. Scale bars = 100 µm.

https://doi.org/10.7554/eLife.48689.012
Our model of tendon growth shows significant proliferation prior to P30 that coincides with expansion and maturation of the tendon matrix.

By P30, tendon cells transition from relatively high to very low rates of proliferation, signifying a transition from growth to physiological tissue maintenance. Others have shown that matrix maturation and expansion continue after P30, but these changes do not involve proliferation-driven cell growth or longitudinal growth of the tendon.

https://doi.org/10.7554/eLife.48689.020

Videos

Video 1
Movie of sagittal optical sections taken using 2-photon microscopy of P0 Achilles tendon with Hoechst+ nuclei (white) and SHG signal (purple) corresponding to Figure 5A’.
https://doi.org/10.7554/eLife.48689.015
Video 2
Movie of sagittal optical sections taken using 2-photon microscopy of P7 Achilles tendon with Hoechst+ nuclei (white) and SHG signal (purple) corresponding to Figure 5B’.
https://doi.org/10.7554/eLife.48689.016
Video 3
Movie of sagittal optical sections taken using 2-photon microscopy of P14 Achilles tendon with Hoechst+ nuclei (white) and SHG signal (purple) corresponding to Figure 5C’.
https://doi.org/10.7554/eLife.48689.017
Video 4
Movie of sagittal optical sections taken using 2-photon microscopy of P28 Achilles tendon with Hoechst+ nuclei (white) and SHG signal (purple) corresponding to Figure 5D’.
https://doi.org/10.7554/eLife.48689.018
Video 5
Movie of optical sections taken using 2-photon microscopy of P28 Achilles tendon with Scx-GFP+ cells (yellow) and SHG signal (purple).

For this movie, the ‘reslice’ feature in the FIJI software was used to convert the image from the sagittal to transverse view.

https://doi.org/10.7554/eLife.48689.019

Tables

Key resources table
Reagent type
(species) or
resource
DesignationSource or
reference
IdentifiersAdditional
information
Strain, strain background (Mus musculus)Col1a1:tetO-H2B-GFP; ROSA:rtTA (H2B-GFP)Brack, Hochedlinger labs, PMID: 24715455, 19060879
Strain, strain background (Mus musculus)Scx-GFPSchweitzer lab, PMID: 20059955
Strain, strain background (Mus musculus)Scx-CreSchweitzer lab,PMID: 11585810
Strain, strain background (Mus musculus)Gt(ROSA)26Sortm9(CAG.tdTomato)Hze (ai9)/TdTomJacksoncat# 007909
AntibodyRat anti Mouse CD31BDCat#551262(1:100)
AntibodyRat anti Mouse CD45BDCat# 557659(1:100)
AntibodyMouse monoclonal BrdUBiolegendCat# 339808(1:100)
AntibodyRat monoclonal BrdUAbcamCat# 6326(1:100)
Commercial assay or kitGreen Flow Cytometry Reference BeadsMolecular ProbesCat# C16508
Commercial assay or kitClick-iT EdUInvitrogenCat# C10337
Commercial assay or kitQubit HS RNA assayInvitrogenCat# Q32852
Commercial assay or kitSuperScriptThermo FisherCat#18091050
Commercial assay or kitSYBRApplied BiosystemsCat#4367659
Chemical compound, drugDoxSigmacat#D9891
Chemical compound, drugCollagenase IIWorthingtonCat# LS004176
Chemical compound, drugDMEMGibcoCat#11956–092
Chemical compound, drugP/SCorningCat#30002 CL
Chemical compound, drugHepesGibcoCat#15630–80
Chemical compound, drugCollagenase IGibcoCat# 17100–017
Chemical compound, drugDispaseGibcoCat# 1710541
Chemical compound, drugBrdUSigmaCat#B5002
Chemical compound, drugHoechstThrmo FisherCat# H3569
Chemical compound, drugTRIzolInvitrogenCat# 15596026
Software, algorithmPrism8GraphPad
Software, algorithmR'stats' version 3.5.1(Team)

Data availability

All data generated or analyzed in this study are included in the manuscript and supporting files. Source data and R code have been provided for Figure 4.

Additional files

Source code 1

RT-qPCR data analysis R script.

https://doi.org/10.7554/eLife.48689.021
Source code 2

Rmarkdown file for generating RT-qPCR statistics tables.

https://doi.org/10.7554/eLife.48689.022
Supplementary file 1

RT-qPCR Statistics tables for Figure 4.

https://doi.org/10.7554/eLife.48689.023
Supplementary file 2

List of RT-qPCR primers used for Figure 4.

https://doi.org/10.7554/eLife.48689.024
Transparent reporting form
https://doi.org/10.7554/eLife.48689.025

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