High and asymmetric somato-dendritic coupling of V1 layer 5 neurons independent of visual stimulation and locomotion
- University of Edinburgh, United Kingdom
Figures
Figure 1 with 2 supplements
Highly correlated activity in the whole apical tuft of layer 5 pyramidal neurons.
(A) Schematic of the methodological approach. Calcium transients were recorded either in multiple apical tuft dendrites in a single focal plane, or semi-simultaneously in different compartments of …
Figure 1—figure supplement 1
Single spine calcium transients in the apical tuft dendrites of layer 5 neurons.
(A) Two-photon image of an example field of view showing the apical tuft branches of an individual GCaMP6-labelled layer 5 neuron. (B) Example ΔF/F0 traces for the individual spines shown in panel …
Figure 1—figure supplement 2
Impact of different thresholds and coincident windows on the quantification of branch-specific and compartment-specific events.
(A) Example ΔF/F0 traces of two sibling branches in the apical tuft branches of an individual layer 5 pyramidal neuron. Asterisks indicate two calcium transients which were not detected using the …
Figure 2 with 2 supplements
High correlation of calcium transients between neuronal compartments (soma, trunk, apical tuft) of individual layer 5 neurons.
(A) Anatomical reconstruction of an individual GCaMP6s-labeled layer 5 pyramidal neuron imaged at two focal planes semi-simultaneously (red dotted lines, proximal; blue, distal). Left panel, coronal …
Figure 2—figure supplement 1
Scatter plots of peak amplitudes of individual calcium transients in all proximal and distal compartments imaged semi-simultaneously.
Each dot represents a calcium transient. Peak amplitudes were normalized to the maximum amplitude in each compartment. x-axis: proximal compartment; y-axis: distal compartment. Black line indicates …
Figure 2—figure supplement 2
Most dendritic events detected as branch-specific were also detected in the trunk.
(A) Example ΔF/F0 traces of GCaMP6s calcium transients imaged semi-simultaneously in the trunk (red) and in two sibling apical tuft branches (black) of an individual layer 5 neuron. The asterisks …
Figure 3 with 1 supplement
Frequency of calcium transients decreases in a distance and amplitude-dependent dependent manner from the soma to the apical tuft.
(A) Schemata of the neuronal compartments imaged simultaneously in individual layer 5 neurons. (B) Left panel, representative ΔF/F0 traces of GCaMP6s calcium transients imaged semi-simultaneously in …
Figure 3—figure supplement 1
Frequency decrease of calcium transients from soma to apical tuft.
(A) Histogram distribution of the depths at which somata of neurons included in this study have been imaged. The red dotted line indicates the median (528 μm from the dura). (B) Estimation of the …
Figure 4 with 1 supplement
Ex vivo calibration of GCaMP6s and GCaMP6f signals in soma and apical tuft dendrites.
(A) Experimental schemata. Layer 5 pyramidal neurons expressing GCaMP6s or GCaMP6f were recorded using whole-cell patch clamp in acute cortical slices of the primary visual cortex. An example neuron …
Figure 4—figure supplement 1
Ex vivo calibration of GCaMP6s in soma and apical tuft dendrites across subthreshold and suprathreshold dendritic stimulation parameters.
(A) Experimental schemata. Layer 5 pyramidal neurons expressing GCaMP6s were recorded using whole-cell patch clamp in acute cortical slices of the primary visual cortex. The soma and distal apical …
Figure 5 with 2 supplements
Locomotion and visual stimulation do not alter the relationship between somatic and dendritic calcium transients in layer 5 pyramidal neurons.
(A) Mean proportion of compartment-specific calcium transients as a function of calcium transient amplitude (average of data points from Figure 3C (i–iv)). In red, proportion of events only detected …
Figure 5—figure supplement 1
Behavioural-state transitions between stationary and locomotion do not alter the relationship between somatic and dendritic calcium transients.
(A) Proportion of compartment-specific events detected in the proximal compartment and not in the distal one, as a function of calcium transient amplitude, during periods of visual stimulation (left …
Figure 5—figure supplement 2
Proportion of coincident calcium transients in proximal and distal compartments imaged semi-simultaneously during the different behavioural conditions: visual stimulation (stim), darkness (dark), stationary (still) and locomotion (loco) periods.
Each data point corresponds to one neuron. No significant difference was found across conditions (Repeated Measures Two-way ANOVA on log-transformed data, p=0.43, p=0.29 and p=0.35 for visual …
Figure 6
Orientation selectivity does not alter the relationship between somatic and dendritic calcium transients in layer 5 pyramidal neurons.
(A) Morphological reconstruction (left) and two-photon image (right) of apical tuft branches of one individual neuron imaged during the presentation of drifting gratings. (B) Example GCaMP6s …
Videos
Video 1
Two-photon imaging movie showing calcium signals detected in the soma and not detected in the corresponding trunk of an individual layer 5 pyramidal neuron in V1.
Left panel, two-photon calcium imaging (raw data) for a pair of neuronal compartments (Soma-Trunk) imaged semi-simultaneously. Both the soma (lower quadrant, circled in red) and the corresponding …
Video 2
Two-photon imaging movie showing calcium signals detected in the apical trunk and not detected in the corresponding tuft of an individual layer 5 pyramidal neuron in V1.
Left panel, two-photon calcium imaging (raw data) for a pair of neuronal compartments (Trunk-Tuft) imaged semi-simultaneously. Both the apical trunk (lower quadrant, circled in red) and the …
