(a) Schematic representation of IL-6 receptor assembly kinetics elicited by IL-6 wildtype (top panel), HyIL-6 (middle panel) and IL-6 mutants molecules (bottom panel). (b) Crystal structure of IL-6, …
(a) Blow out of the site-2 (bottom panel) and site-3 (top panel) binding interface of the IL-6 hexameric complex. IL-6Rα and gp130 exhibits extensive contacts across both interfaces. (b) Amino acid …
(a–b) HeLa cells were stimulated with the indicated doses of IL-6 ligands for 15 min and levels of STAT1 (a) and STAT3 (b) were analyzed by phospho-flow cytometry. Sigmoidal curves were fitted with …
(a) HepG2 cells were pretreated with 10 µg/ml Tocilizumab (Tcz) as indicated for 30 min and then stimulated with 100 nM of the different IL-6 variants for 15 min. Phosphorylation of STAT3 was …
(a) Quantification of gp130 homodimerization in the plasma membrane by dual-color single-molecule co-localization/co-tracking. mXFP-gp130 was expressed in RPE1 gp130KO cells and labeled via anti-GFP …
(a) Characterization of gp130KO HeLa and RPE1 by dose-response experiments with HyIL-6. (b) RPE1 gp130ko cell transfected with XFP-GP130 and cell surface labeled by antiGFP-NBDY647 and antiGFP-NBRHO1…
(a) Trajectories (RHO11 and DY647 and co-trajectories) of representative cells, stimulated with the indicated IL-6 mutants. (b) Mean-square-displacement batch analysis (2–10 steps,>20 cells each) of …
(a–b) HeLa cells were stimulated with saturating concentrations of the different IL-6 ligands for the indicated times in the presence of CHX to block new protein synthesis. Levels of gp130 were …
(a) HeLa cells were transfected with different concentrations of siRNA targeting clathrin for 24 or 48 hr. Levels of clathrin were measured by western blot using clathrin specific antibodies. GAPDH …
(a) Schematic representation of the different chimera receptors designed for this study. IL-27Rα intracellular domain was swapped for that of gp130 or a truncated version of the latter lacking all …
(a) The different RPE1 stable clones were stimulated with saturating concentrations of HyIL-6 for the indicated times and the levels of STAT1 (left panel) or STAT3 (right panel) activation were …
(a) volcano plot showing significant genes differently expressed in Th1 cells after 6 hr stimulation with HyIL6. The red dash lines demark fold change = 1.5. (b) scatter plot showing mean gene …
(a–c) Human CD4 T cells were isolated from buffy coats and differentiated into Th1 cells for five days. 24 hr before stimulation, cells were starved of cytokines. Starved-Th1 cells were stimulated …
(a) Human CD4 T cells were isolated from whole PBMCs and treated with Treg polarizing conditions in the presence of saturating concentrations of the different IL-6 variants for five days. Percentage …
Number of STAT3 binding sites.