Recombinant DNA reagent | rfng (zebrafish, Danio rerio) in situ hybridisation probe | Cheng et al., 2004, PMID: 15068793 | | |
Recombinant DNA reagent | wnt1 (zebrafish) in situ hybridisation probe | Molven et al., 1991, PMID: 2009859 | | |
Recombinant DNA reagent | sema3gb (zebrafish) in situ hybridisation probe | Terriente et al., 2012, PMID: 22764046 | | |
Recombinant DNA reagent | egr2b (zebrafish) in situ hybridisation probe | Oxtoby and Jowett, 1993, PMID: 8464695 | | |
Sequence-based reagent | rfng (zebrafish) HCR probe | Molecular Instruments | | Designed and supplied by Molecular Instruments |
Sequence-based reagent | wnt1 (zebrafish) HCR probe | Molecular Instruments | | Designed and supplied by Molecular Instruments |
Sequence-based reagent | epha4 (zebrafish) HCR probe | Molecular Instruments | | Designed and supplied by Molecular Instruments |
Antibody | anti-pMLC (rabbit polyclonal) | Cell Signalling Technology | Cat # 3671 RRID:AB_330248 | 1:200 |
Antibody | anti-Taz (rabbit monoclonal) | Cell Signalling Technology | Cat # D24E4 RRID:AB_10950494 | 1:200 |
Antibody | anti-EphA4 (rabbit polyclonal) | Irving et al., 1996, PMID: 8575627 | | 1:450 |
Chemical compound, drug | phalloidin-atto 647N | Sigma-Aldrich | Cat # 65906 | 1:250 |
Chemical compound, drug | blebbistatin | Sigma-Aldrich | Cat # B0560 | 12.5 μM |
Chemical compound, drug | latrunculinB | Sigma-Aldrich | Cat # 428020 | 50 nM |
Sequence-based reagent | morpholino to block mypt1 | Gutzman and Sive, 2010, PMID: 20147380 | | ATTTTTTGTGACTTACTCAGCGATG |
Sequenced-based reagent | morpholino to block taz | Hong et al., 2005, PMID: 16099986 | | CTGGAGAGGATTACCGCTCATGGTC |
Gequenced-based reagent | morpholino to block yap1 | Skouloudaki et al., 2009, PMID: 19439659 | | AGCAACATTAACAACTCACTTTAGG |
Genetic reagent (D. rerio) | epha4a null mutant | This study | | Sequence in Figure 2—figure supplement 1 |
Genetic reagent (D. rerio) | epha4a Δ651 mutant | This study | | Sequence in Figure 2—figure supplement 1 |
Genetic reagent (D. rerio) | epha4a KD mutant | This study | | Sequence in Figure 2—figure supplement 1 |
Genetic reagent (D. rerio) | epha4a ΔPDZBD mutant | This study | | Sequence in Figure 2—figure supplement 1 |
Sequence-based reagent | genomic target of gRNA to create epha4a null mutant | This study | | GGCTGATGAAAGCTTCACGC |
Sequence-based reagent | forward primer to screen for epha4a null mutant | This study | | GCTCCGCAGTACATTTTAGGG |
Sequence-based reagent | reverse primer to screen for epha4a null mutant | This study | | GTCTTTCCTCTCACAGTGGGA |
Sequence-based reagent | genomic target of gRNA to create epha4a Δ651 mutant | This study | | GGAAAGCGTGAGATCTGTG |
Sequence-based reagent | forward primer to screen for epha4a Δ651 mutant | This study | | CCCTTCACATACGAGGACCCC |
Sequence-based reagent | reverse primer to screen for epha4a Δ651 mutant | This study | | GCTCGCTCACATTCAACACA |
Sequence-based reagent | genomic target of gRNA to create epha4a KD mutant | This study | | GGAAAGCGTGAGATCTGTG |
Sequence-based reagent | Donor oligonucleotide to create epha4a KD mutant | This study | | AAGATGCCTGGAAAGCGTGAaATtTGcGTGGCCATAAAAACCCTAAtGGCAGGgTACACCGACAAGCAAAGGCG |
Sequence-based reagent | forward primer to screen for epha4a KD mutant | This study | | CCCTTCACATACGAGGACCCC |
Sequence-based reagent | reverse primer to screen for epha4a KD mutant | This study | | GCTCGCTCACATTCAACACA |
Sequence-based reagent | genomic target of gRNA to create epha4a ΔPDZBD mutant | This study | | GCAGCAAATGCAGGACAGGA |
Sequence-based reagent | forward primer to screen for epha4a ΔPDZBD mutant | This study | | AGTTCTCCCCCTCAAACAAAA |
Sequence-based reagent | reverse primer to screen for epha4a ΔPDZBD mutant | This study | | CAGTACAGCGCTAAACGATCC |
Sequence-based reagent | genomic targets of gRNAs to create deletions in mypt1 | This study | | GGTACGGTACGAAAGAGAGG ACGAAGGTGAAGTTCGACGA GGAACGAGCAGTTAAAGCGC CTGCTCGAGCGGAGACACGG TGGCGGACGCCAAGCAGAAG |
Sequence-based reagent | forward primer to screen for deletions in mypt1 | This study | | CGACGTAACCAGGTTTGTTCA |
Sequence-based reagent | reverse primer to screen for deletions in mypt1 | This study | | ACATTGGCGTAGTTGATGTCG |
Sequence-based reagent | genomic targets of gRNAs to create deletions in yap1 | This study | | CTCAACCTCATCGGCACGGA CCCGAACATGGACGATCTGG AAGAGCCTCCAGATCGGTCT |
Sequence-based reagent | forward primer to screen for deletions in yap1 | This study | | GCCGGACACAGAACATCTTTT |
Sequence-based reagent | reverse primer to screen for deletions in yap1 | This study | | CTGTTTGTGGTTTCTGAGGGG |
Sequence-based reagent | genomic targets of gRNAs to create deletions in taz | This study | | CAAAGACCTGGACACGGATC GAGATGGCCTTCACCCCCAA GGAGACTCCACTCCCACACC |
Sequence-based reagent | forward primer to screen for deletions in taz | This study | | AAAACTCTCCAAACTCCACGC |
Sequence-based reagent | reverse primer to screen for deletions in taz | This study | | CCGTGTTCAATACTCATTCCCC |
Sequence-based reagent | genomic targets of gRNAs to create deletions in tead1a | This study | | TAAGCCCATGGACAATGACG TGACATTGAGCAGAGCTTTC CATTCTCTCAATGTCCTCCC |
Sequence-based reagent | forward primer to screen for deletions in tead1a | This study | | GTCAGTGTGCCTTGAGTTCTC |
Sequence-based reagent | reverse primer to screen for deletions in tead1a | This study | | ATTTTGCCCTCATCAGACAGG |
Sequence-based reagent | genomic targets of gRNAs to create deletions in tead3 | This study | | CATTGAACAAAGCTTCCAGG CTGAGAGGATGATCTTTCTG ATGGACAAAACCGGAATGGA |
Sequence-based reagent | forward primer to screen for deletions in tead3 | This study | | GAGCCGCCACCATTGCAG |
Sequence-based reagent | reverse primer to screen for deletions in tead3 | This study | | TAGCTCTGACTAACGTGGGTG |
Sequence-based reagent | RVD sequences of ephrinb3b TALENs | This study | | Left: NG HD NN NN NN NN NI NG NG NG HD NI NI NI NG NN NN HD Right: HD NI NN NN NI NN NI NI NG NG HD HD HD NI NI NG HD HD NI NG |