The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast
Abstract
Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
Biotechnology and Biological Sciences Research Council (BB/P019706/1)
- Matthew C Whitby
Wellcome (090767/Z/09/Z)
- Matthew C Whitby
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Maria Spies, University of Iowa, United States
Publication history
- Received: June 29, 2019
- Accepted: December 18, 2019
- Accepted Manuscript published: December 19, 2019 (version 1)
- Version of Record published: January 9, 2020 (version 2)
Copyright
© 2019, Wong et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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