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Mechanisms underlying the response of mouse cortical networks to optogenetic manipulation

  1. Alexandre Mahrach
  2. Guang Chen
  3. Nuo Li
  4. Carl van Vreeswijk
  5. David Hansel  Is a corresponding author
  1. CNRS-UMR 8002, France
  2. Baylor College of Medicine, United States
Research Article
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Cite this article as: eLife 2020;9:e49967 doi: 10.7554/eLife.49967

Abstract

GABAergic Interneurons can be subdivided into three subclasses: parvalbumin positive (PV), somatostatin positive (SOM) and serotonin positive neurons. With principal cells (PCs) they form complex networks. We examine PCs and PV responses in mouse anterior lateral motor cortex (ALM) and barrel cortex (S1) upon PV photostimulation in vivo. In ALM layer 5 and S1, the PV response is paradoxical: photoexcitation reduces their activity. This is not the case in ALM layer 2/3. We combine analytical calculations and numerical simulations to investigate how these results constrain the architecture. Two-population models cannot explain the results. Four-population networks with V1-like architecture account for the data in ALM layer 2/3 and layer 5. Our data in S1 can be explained if SOM neurons receive inputs only from PCs and PV neurons. In both four-population models, the paradoxical effect implies not too strong recurrent excitation. It is not evidence for stabilization by inhibition.

Article and author information

Author details

  1. Alexandre Mahrach

    Integrative Neuroscience and Cognition Center, CNRS-UMR 8002, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  2. Guang Chen

    Department of Neuroscience, Baylor College of Medicine, Houston, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Nuo Li

    Department of Neuroscience, Baylor College of Medicine, Houston, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Carl van Vreeswijk

    Integrative Neuroscience and Cognition Center, CNRS-UMR 8002, Paris, France
    Competing interests
    The authors declare that no competing interests exist.
  5. David Hansel

    Integrative Neuroscience and Cognition Center, CNRS-UMR 8002, Paris, France
    For correspondence
    david.hansel@parisdescartes.fr
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1352-6592

Funding

Agence Nationale de la Recherche (14-NEUC-0001-01)

  • Carl van Vreeswijk

Agence Nationale de la Recherche (13-BSV4-0014-02)

  • David Hansel

Agence Nationale de la Recherche (09-SYSC-002-01)

  • David Hansel

Helen Hay Whitney Foundation

  • Nuo Li

Robert and Janice McNair Foundation

  • Nuo Li

Alfred P. Sloan Foundation

  • Nuo Li

National Institutes of Health (NS104781)

  • Nuo Li

Pew Charitable Trusts

  • Nuo Li

Simons Collaboration on the Global Brain (543005)

  • Nuo Li

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All procedures were in accordance with protocols approved by the Janelia Research Campus and Baylor College of Medicine Institutional Animal Care and Use Committee.

Reviewing Editor

  1. David Kleinfeld, University of California, San Diego, United States

Publication history

  1. Received: July 5, 2019
  2. Accepted: December 25, 2019
  3. Accepted Manuscript published: January 17, 2020 (version 1)
  4. Version of Record published: February 11, 2020 (version 2)

Copyright

© 2020, Mahrach et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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