(a) Seven library plasmids were high-copy suppressors of CidBwPip toxicity. Red genes suppressed when individually sub-cloned. Library plasmid YGPM25o01 includes URA3 and measures screen efficiency since it is an expected suppressor; Backslashes and brackets denote ORF truncations. (b) Five-fold serial dilutions of yeast (W303-1A) with recovered suppressing library plasmids co-transformed with pRS416GAL1-CidB3xFLAG-wPip. Library plasmid suppression varied. Suppression by YGPM25o01 (URA3 control), YGPM26g16, and YGPM32e11 was strong and consistent (three replicates). Plasmids YGPM12h13, YGPM21f02, YGPM32b05, and YGPM11h18, showed weaker and less consistent suppression across four replicates. (c) Individual yeast genes SRP1, RTT103, and HRP1 suppressed CidBwPip toxicity (three replicates). (d) Immunoblot analysis confirmed that suppressor plasmids do not reduce CidB expression. CidB and suppressors were controlled by GAL1 and endogenous promoters, respectively. Asterisk, an unknown cross-reacting yeast protein. Ponceau S staining indicated relative sample loading.