(A) Schematic representation of murine male germ cells transitioning from round spermatids to elongated spermatozoa. These steps occur within the testis seminiferous epithelium and are conserved in …
(A) Hipk4 expression in various murine tissues as determined by qPCR analysis of the Origene TissueScan Mouse Normal cDNA array. Data are normalized to Gapdh. (B–C) Hipk4 expression in adult mouse (B…
Hipk4 transcript labeling by in situ hybridization in formalin-fixed, paraffin-embedded testis sections from wild-type C57BL/6NJ mice of the designated ages. Arrowheads indicate the loss of Hipk4 …
(A-B) Immunofluorescence images of cryosectioned testes from adult wild-type (A) and HIPK4 knockout (B) mice. Stages for each testis section are shown. Scale bars: 20 µm.
(A) Concentrations of epididymal sperm ± s.d. P values for the indicated statistical comparison are shown. (B) Percentage of epididymal sperm that were motile ± s.d. P values for the indicated …
Phase contrast images of sperm obtained from mice with the designated genotypes. Sperm were treated with Diff-Quik stain prior to imaging, and multiple examples of homozygous mutant sperm are shown …
(A) Western blot detection of soluble phosphotyrosine-containing proteins in sperm before or after capacitation with TYH medium + BSA (Cap buffer). (B) Percentage of wild-type or Hipk4 knockout …
(A,A’) PAS-stained sections of seminiferous tubules from WT or Hipk4-/- mice at stages IX-XII of spermatogenesis. Green arrowheads point to the heads of representative elongating spermatids (Step …
(A-B) Fixed, paraffin-embedded testis sections from WT (A) and Hipk4–/– (B) mice stained with PAS reagents. Stages for the tubules shown in each micrograph are indicated. (C-D) Fixed, …
(A) TEM images of step 4–8 spermatids from adult WT and Hipk4–/– mice. (B) TEM images of step 10 Hipk4–/– spermatids. (C) Higher magnification TEM images of the groove belt region in step 10 WT and H…
(A) Immunofluorescence imaging of the inner nuclear membrane protein DPY19L2 (red) in dissociated spermatids. The acrosome is labeled with FITC-PNA (green), and nuclei are stained with Hoechst 33342 …
(A) Summary of the types of RNA that were increased (up-regulated) or decreased (down-regulated) in Hipk4 knockout testes compared to wild-type, as measured by quadruplicate microarray analysis of …
Testis microarray data comparing WT and HIPK4 KO transcripts .
(A–B) Brightfield images of NIH-3T3 cells retrovirally transduced with FLAG-tagged Hipk4 or kinase-dead Hipk4 K40S. (C) HIPK4-expressing NIH-3T3 cell with multiple nuclei. (D–E) Phalloidin and …
Phosphoproteome of HIPK4-expressing NIH-3T3 cells.
(A–C) Fluorescence imaging of enzymatically dissociated spermatids co-labeled with FITC-PNA and (A) anti-bβ actin, (B) anti-CAPZA3, or (C) anti-CAPZB3. Nuclei were stained with Hoeschst 33342. …
(A) Cryosectioned seminiferous tubules (stage X/XI) stained with Alexa Fluor-647-phalloidin to stain F-actin and an Alexa Fluor-488-anti-EB3 antibody to stain the manchette. Basal and apical …
Spermatids were isolated from the testes of wild-type and Hipk4-/- mice, fixed, and then stained with a rabbit polyclonal anti-β-actin antibody and Alexa Fluor 488-conjugated anti-rabbit IgG …
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